Time of neuron origin and gradients of neurogenesis in midbrain dopaminergic neurons in the mouse

Shirley A. Bayer, Katherine V. Wills, Lazaros C. Triarhou, Bernardino Ghetti

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Previous [3H]thymidine studies in Nisslstained sections in rats established that the substantia nigra pars compacta and the ventral tegmental area originate sequentially according to an anterolateral to posteromedial neurogenetic gradient. We investigated whether that same pattern is found in mice in the dopaminergic neurons in each of these structures. Using tyrosine hydroxylase immunostaining combined with [3H]thymidine autoradiography, the time of origin of dopaminergic midbrain neurons in the retrorubral field, the substantia nigra pars compacta, the ventral tegmental area, and the interfascicular nucleus was determined in postnatal day 20 mice. The dams of the experimental animals were injected with [3H]thymidine on embryonic days (E) 11-E12, E12-E13, E13-E14, and E14-E15. The time of origin profiles for each group indicated significant differences between populations. The retrorubral field and the substantia nigra pars compacta arose nearly simultaneously and contained the highest proportion of neurons, 49 to 37%, generated on or before E11. Progressively fewer early-generated neurons were found in the ventral tegmental area (20%), and the interfascicular nucleus (8.5%). In addition, anterior dorsolateral neurons in the substantia nigra and ventral tegmental area were more likely to be generated early than the posterior ventromedial neurons. These findings indicate that mouse and rat brains have nearly identical developmental patterns in the midbrain, and neurogenetic gradients in dopaminergic neurons are similar to those found in Nissl studies in rats.

Original languageEnglish
Pages (from-to)191-199
Number of pages9
JournalExperimental Brain Research
Volume105
Issue number2
DOIs
StatePublished - Aug 1995

Fingerprint

Ventral Tegmental Area
Dopaminergic Neurons
Neurogenesis
Mesencephalon
Thymidine
Neurons
Raphe Nuclei
Tyrosine 3-Monooxygenase
Substantia Nigra
Autoradiography
Brain
Population
Pars Compacta

Keywords

  • [H]Thymidine autoradiography
  • Interfascicular nucleus
  • Mouse
  • Retrorubral field
  • Substantia nigra pars compacta
  • Ventral tegmental area

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Time of neuron origin and gradients of neurogenesis in midbrain dopaminergic neurons in the mouse. / Bayer, Shirley A.; Wills, Katherine V.; Triarhou, Lazaros C.; Ghetti, Bernardino.

In: Experimental Brain Research, Vol. 105, No. 2, 08.1995, p. 191-199.

Research output: Contribution to journalArticle

Bayer, Shirley A. ; Wills, Katherine V. ; Triarhou, Lazaros C. ; Ghetti, Bernardino. / Time of neuron origin and gradients of neurogenesis in midbrain dopaminergic neurons in the mouse. In: Experimental Brain Research. 1995 ; Vol. 105, No. 2. pp. 191-199.
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