Time-Resolved Fluorescence Spectroscopy of the Retinal Pigment Epithelium: Age-Related Studies

Rinaldo Cubeddu, Franco Docchio, Roberta Ramponi, Mike Boulton

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Time-resolved fluorescence spectroscopy shows promise as a high-sensitivity, high-discrimination tool for diagnostic purposes. In ophthalmology, the application of this technique to the study and the diagnosis of aging processes within ocular tissues is presently investigated. An age-related study of the pigments (melanin, lipofuscin, and melanolipofuscin) of the retinal pigment epithelium, performed using time-resolved fluorescence spectroscopy with picosecond gating, is herein presented. The study described in the present paper was performed on intact pigment granules, extracted with soft procedures, and the results have been correlated with the ultrastructure of the granules, and with results of continuous wave absorption and fluorescence spectroscopy. Both classes of pigments exhibit age-related fluorescence properties. These changes may be detected using continuous wave fluorescence spectroscopy techniques, but are greatly enhanced when gating techniques are used. Melanin, in particular, exhibits age-related spectral modifications which suggest a progressive accumulation of lipofuscin-like fluorophores in the granule. The implications of this paper in relation to the possibility of monitoring the fluorescence of these pigments in vivo are discussed.

Original languageEnglish (US)
Pages (from-to)2218-2225
Number of pages8
JournalIEEE Journal of Quantum Electronics
Volume26
Issue number12
DOIs
StatePublished - Dec 1990

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ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

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