Tips and techniques for processing and sectioning undecalcified murine bone specimens

Thomas B. Bemenderfer, Jonathan S. Harris, Keith W. Condon, Melissa Kacena

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Preparation of mineralized tissue specimens for bone-specific staining encompasses a critical sequence of histological techniques that provides visualization of tissue and cellular morphology. Bone specimens are fixed in 10 % neutral-buffered formalin, dehydrated in graded ethanol (EtOH) solutions (and optionally cleared in xylene), infiltrated and embedded in polymethyl methacrylate (methyl methacrylate), classically sliced into 4-10 micrometer (μm) sections, and stained with bone-specific histological stains such as von Kossa (with either nuclear fast red solution counterstain or MacNeal's tetrachrome counterstain), modified Goldner's trichrome, and alizarin red S stain. Here, we describe the tissue processing of mineralized mouse bones from dissection to staining for histological analysis by light microscopy.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages123-147
Number of pages25
Volume1130
ISBN (Print)9781627039888
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1130
ISSN (Print)10643745

Fingerprint

Bone and Bones
Coloring Agents
Staining and Labeling
Histological Techniques
Xylenes
Methacrylates
Polymethyl Methacrylate
Formaldehyde
Dissection
Microscopy
Ethanol
Light
nuclear fast red
Alizarin Red S

Keywords

  • Bone
  • Dehydration
  • Embedding
  • Fixation
  • Methyl methacrylate
  • Sectioning
  • Staining

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bemenderfer, T. B., Harris, J. S., Condon, K. W., & Kacena, M. (2014). Tips and techniques for processing and sectioning undecalcified murine bone specimens. In Methods in Molecular Biology (Vol. 1130, pp. 123-147). (Methods in Molecular Biology; Vol. 1130). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-989-5_10

Tips and techniques for processing and sectioning undecalcified murine bone specimens. / Bemenderfer, Thomas B.; Harris, Jonathan S.; Condon, Keith W.; Kacena, Melissa.

Methods in Molecular Biology. Vol. 1130 Humana Press Inc., 2014. p. 123-147 (Methods in Molecular Biology; Vol. 1130).

Research output: Chapter in Book/Report/Conference proceedingChapter

Bemenderfer, TB, Harris, JS, Condon, KW & Kacena, M 2014, Tips and techniques for processing and sectioning undecalcified murine bone specimens. in Methods in Molecular Biology. vol. 1130, Methods in Molecular Biology, vol. 1130, Humana Press Inc., pp. 123-147. https://doi.org/10.1007/978-1-62703-989-5_10
Bemenderfer TB, Harris JS, Condon KW, Kacena M. Tips and techniques for processing and sectioning undecalcified murine bone specimens. In Methods in Molecular Biology. Vol. 1130. Humana Press Inc. 2014. p. 123-147. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-989-5_10
Bemenderfer, Thomas B. ; Harris, Jonathan S. ; Condon, Keith W. ; Kacena, Melissa. / Tips and techniques for processing and sectioning undecalcified murine bone specimens. Methods in Molecular Biology. Vol. 1130 Humana Press Inc., 2014. pp. 123-147 (Methods in Molecular Biology).
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