Tips and techniques for processing and sectioning undecalcified murine bone specimens

Thomas B. Bemenderfer, Jonathan S. Harris, Keith W. Condon, Melissa A. Kacena

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Preparation of mineralized tissue specimens for bone-specific staining encompasses a critical sequence of histological techniques that provides visualization of tissue and cellular morphology. Bone specimens are fixed in 10 % neutral-buffered formalin, dehydrated in graded ethanol (EtOH) solutions (and optionally cleared in xylene), infiltrated and embedded in polymethyl methacrylate (methyl methacrylate), classically sliced into 4-10 micrometer (μm) sections, and stained with bone-specific histological stains such as von Kossa (with either nuclear fast red solution counterstain or MacNeal's tetrachrome counterstain), modified Goldner's trichrome, and alizarin red S stain. Here, we describe the tissue processing of mineralized mouse bones from dissection to staining for histological analysis by light microscopy.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages123-147
Number of pages25
Volume1130
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1130
ISSN (Print)10643745

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bemenderfer, T. B., Harris, J. S., Condon, K. W., & Kacena, M. A. (2014). Tips and techniques for processing and sectioning undecalcified murine bone specimens. In Methods in Molecular Biology (Vol. 1130, pp. 123-147). (Methods in Molecular Biology; Vol. 1130). https://doi.org/10.1007/978-1-62703-989-5-10