Tissue and gene specificity of mouse renin expression

Loren Field, Ross A. McGowan, Douglas P. Dickinson, Kenneth W. Gross

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

The Ren-1 locus of mice encodes the protease renin, which with converting enzyme processes angiotensinogen to the potent vasopressor angiotensin II. Some strains of mice appear to carry a duplication of the renin structural gene (Ren-2) near the Ren-1 locus. Strains with the gene duplication can exhibit as much as 100-fold higher levels of submaxillary gland renin compared to strains with a single gene copy. In contrast, kidney renin levels appear to be unaffected by the gene duplication. Sequence analysis of a 319 bp renin cDNA recombinant isolated from a kidney library from the two-gene strain DBA/2Ha corresponds to a transcript of the Ren-1 gene. Moreover, a single base substitution of A for G at residue #996 in the kidney renin mRNA creates a potential glycosylation recognition site that may, in part, account for the differential glvcosylation of kidney and submaxillary gland renins. In addition, our tissue surveys indicate that mature mRNAs from the Ren loci are detectable in adrenal gland and testes, as well as sublingual and parotid salivary glands, and reveal length variation for the renin transcripts in at least the submaxillary gland.

Original languageEnglish (US)
Pages (from-to)597-603
Number of pages7
JournalHypertension
Volume6
Issue number4
StatePublished - 1984
Externally publishedYes

Fingerprint

Organ Specificity
Renin
Submandibular Gland
Genes
Kidney
Gene Duplication
Angiotensinogen
Messenger RNA
Parotid Gland
Adrenal Glands
Salivary Glands
Gene Library
Glycosylation
Angiotensin II
Sequence Analysis
Testis
Peptide Hydrolases
Complementary DNA
Enzymes

Keywords

  • Differential regulation
  • Gene duplication
  • Transcript length variation

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Field, L., McGowan, R. A., Dickinson, D. P., & Gross, K. W. (1984). Tissue and gene specificity of mouse renin expression. Hypertension, 6(4), 597-603.

Tissue and gene specificity of mouse renin expression. / Field, Loren; McGowan, Ross A.; Dickinson, Douglas P.; Gross, Kenneth W.

In: Hypertension, Vol. 6, No. 4, 1984, p. 597-603.

Research output: Contribution to journalArticle

Field, L, McGowan, RA, Dickinson, DP & Gross, KW 1984, 'Tissue and gene specificity of mouse renin expression', Hypertension, vol. 6, no. 4, pp. 597-603.
Field L, McGowan RA, Dickinson DP, Gross KW. Tissue and gene specificity of mouse renin expression. Hypertension. 1984;6(4):597-603.
Field, Loren ; McGowan, Ross A. ; Dickinson, Douglas P. ; Gross, Kenneth W. / Tissue and gene specificity of mouse renin expression. In: Hypertension. 1984 ; Vol. 6, No. 4. pp. 597-603.
@article{d0fb292e7a1a45978663e20edeadbabd,
title = "Tissue and gene specificity of mouse renin expression",
abstract = "The Ren-1 locus of mice encodes the protease renin, which with converting enzyme processes angiotensinogen to the potent vasopressor angiotensin II. Some strains of mice appear to carry a duplication of the renin structural gene (Ren-2) near the Ren-1 locus. Strains with the gene duplication can exhibit as much as 100-fold higher levels of submaxillary gland renin compared to strains with a single gene copy. In contrast, kidney renin levels appear to be unaffected by the gene duplication. Sequence analysis of a 319 bp renin cDNA recombinant isolated from a kidney library from the two-gene strain DBA/2Ha corresponds to a transcript of the Ren-1 gene. Moreover, a single base substitution of A for G at residue #996 in the kidney renin mRNA creates a potential glycosylation recognition site that may, in part, account for the differential glvcosylation of kidney and submaxillary gland renins. In addition, our tissue surveys indicate that mature mRNAs from the Ren loci are detectable in adrenal gland and testes, as well as sublingual and parotid salivary glands, and reveal length variation for the renin transcripts in at least the submaxillary gland.",
keywords = "Differential regulation, Gene duplication, Transcript length variation",
author = "Loren Field and McGowan, {Ross A.} and Dickinson, {Douglas P.} and Gross, {Kenneth W.}",
year = "1984",
language = "English (US)",
volume = "6",
pages = "597--603",
journal = "Hypertension",
issn = "0194-911X",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Tissue and gene specificity of mouse renin expression

AU - Field, Loren

AU - McGowan, Ross A.

AU - Dickinson, Douglas P.

AU - Gross, Kenneth W.

PY - 1984

Y1 - 1984

N2 - The Ren-1 locus of mice encodes the protease renin, which with converting enzyme processes angiotensinogen to the potent vasopressor angiotensin II. Some strains of mice appear to carry a duplication of the renin structural gene (Ren-2) near the Ren-1 locus. Strains with the gene duplication can exhibit as much as 100-fold higher levels of submaxillary gland renin compared to strains with a single gene copy. In contrast, kidney renin levels appear to be unaffected by the gene duplication. Sequence analysis of a 319 bp renin cDNA recombinant isolated from a kidney library from the two-gene strain DBA/2Ha corresponds to a transcript of the Ren-1 gene. Moreover, a single base substitution of A for G at residue #996 in the kidney renin mRNA creates a potential glycosylation recognition site that may, in part, account for the differential glvcosylation of kidney and submaxillary gland renins. In addition, our tissue surveys indicate that mature mRNAs from the Ren loci are detectable in adrenal gland and testes, as well as sublingual and parotid salivary glands, and reveal length variation for the renin transcripts in at least the submaxillary gland.

AB - The Ren-1 locus of mice encodes the protease renin, which with converting enzyme processes angiotensinogen to the potent vasopressor angiotensin II. Some strains of mice appear to carry a duplication of the renin structural gene (Ren-2) near the Ren-1 locus. Strains with the gene duplication can exhibit as much as 100-fold higher levels of submaxillary gland renin compared to strains with a single gene copy. In contrast, kidney renin levels appear to be unaffected by the gene duplication. Sequence analysis of a 319 bp renin cDNA recombinant isolated from a kidney library from the two-gene strain DBA/2Ha corresponds to a transcript of the Ren-1 gene. Moreover, a single base substitution of A for G at residue #996 in the kidney renin mRNA creates a potential glycosylation recognition site that may, in part, account for the differential glvcosylation of kidney and submaxillary gland renins. In addition, our tissue surveys indicate that mature mRNAs from the Ren loci are detectable in adrenal gland and testes, as well as sublingual and parotid salivary glands, and reveal length variation for the renin transcripts in at least the submaxillary gland.

KW - Differential regulation

KW - Gene duplication

KW - Transcript length variation

UR - http://www.scopus.com/inward/record.url?scp=0021194529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021194529&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 597

EP - 603

JO - Hypertension

JF - Hypertension

SN - 0194-911X

IS - 4

ER -