Tissue distribution and subcellular localization of mammalian myosin I

Mark Wagner, Barbara Barylko, Joseph P. Albanesi

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

Myosin I, a nonfilamentous single-headed actin-activated ATPase, has recently been purified from mammalian tissue (Barylko, B., M. C. Wagner, O. Reizes, and J. P. Albanesi. 1992. Proc. Natl. Acad. Sci. USA. 89:490-494). To investigate the distribution of this enzyme in cells and tissues mAbs were generated against myosin I purified from bovine adrenal gland. Eight antibodies were characterized, five of them (M4-M8) recognize epitope(s) on the catalytic "head" portion of myosin I while the other three (M1-MS) react with the "tail" domain. Immunoblot analysis using antiadrenal myosin I antibody M2 demonstrates the widespread distribution of the enzyme in mammalian tissues. Myosin I was immunolocalized in several cell types including bovine kidney (MDBK), rat kidney (NRK), rat brain, rat phaeochromocytoma (PC12), fibroblast (Swiss 3T3), and CHO cells. In all cases, myosin I was concentrated at the cell periphery. The most intense labeling was observed in regions of the cell usually associated with motile activity (i.e., filopodia, lamellipodia and growth cones). These results are consistent with earlier observations on protozoan myosin I that suggest a motile role for the enzyme at the plasma membrane.

Original languageEnglish (US)
Pages (from-to)163-170
Number of pages8
JournalJournal of Cell Biology
Volume119
Issue number1
StatePublished - Oct 1992
Externally publishedYes

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Myosin Type I
Tissue Distribution
Pseudopodia
Enzymes
Swiss 3T3 Cells
Kidney
Growth Cones
CHO Cells
Antibodies
Pheochromocytoma
Myosins
Adrenal Glands
Tail
Epitopes
Fibroblasts
Head
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology

Cite this

Tissue distribution and subcellular localization of mammalian myosin I. / Wagner, Mark; Barylko, Barbara; Albanesi, Joseph P.

In: Journal of Cell Biology, Vol. 119, No. 1, 10.1992, p. 163-170.

Research output: Contribution to journalArticle

Wagner, M, Barylko, B & Albanesi, JP 1992, 'Tissue distribution and subcellular localization of mammalian myosin I', Journal of Cell Biology, vol. 119, no. 1, pp. 163-170.
Wagner, Mark ; Barylko, Barbara ; Albanesi, Joseph P. / Tissue distribution and subcellular localization of mammalian myosin I. In: Journal of Cell Biology. 1992 ; Vol. 119, No. 1. pp. 163-170.
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