Tissue distribution of fetal liver cells following in utero transplantation in mice

Mark A. Suckow, Amy Zollman, Ivo Cornelissen, Michelle Casad, Julie Roahrig, Francis J. Castellino, Elliot Rosen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Transplantation of hepatic stem cells in utero has been advanced as a potential clinical approach to a variety of diseases, including deficiencies of coagulation factors. Although syngeneic transplantation has met with some success, consideration needs to be given to the potential for transplanted cells to colonize nontarget tissues. Liver cells were harvested from Rosa26 embyros at embryonic age 12.5 days postconception (pc) and transplanted into the peritoneal cavity of syngeneic recipients in utero. Tissues were harvested from tissue recipients at various time points ranging from 1 to 328 days pc, and tissues were stained for β-galactosidase to identify the existence of cells derived from Rosa26 donors. β-galactosidase-positive cells were found in the lung, liver, and brain as early as 20 days pc and through 328 days pc. Positive cells in these tissues existed as islands of cells that were morphologically similar to hepatocytes. In the spleen, individual β-galactosidase-positive cells of both leukocytic and erythrocytic lineages were present, and suggest that hematopoietic cells were transferred to recipients along with hepatocytes. The lack of an inflammatory response to the β-galactosidase-positive cells suggests that the donor cells were immunologically tolerated. In summary, the possibility that cells administered in utero may inadvertently colonize nontarget tissues suggests that clinical application of this method will need to be approached with diligence.

Original languageEnglish (US)
Pages (from-to)860-864
Number of pages5
JournalExperimental Biology and Medicine
Volume230
Issue number11
StatePublished - Dec 2005
Externally publishedYes

Fingerprint

Tissue Distribution
Galactosidases
Liver
Transplantation
Tissue
Cells
Hepatocytes
Blood Coagulation Factors
Stem cells
Brain
Isogeneic Transplantation
Deficiency Diseases
Peritoneal Cavity
Spleen
Lung

Keywords

  • β-galactosidase
  • Hepatocytes
  • Stem cells
  • Transplantation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Suckow, M. A., Zollman, A., Cornelissen, I., Casad, M., Roahrig, J., Castellino, F. J., & Rosen, E. (2005). Tissue distribution of fetal liver cells following in utero transplantation in mice. Experimental Biology and Medicine, 230(11), 860-864.

Tissue distribution of fetal liver cells following in utero transplantation in mice. / Suckow, Mark A.; Zollman, Amy; Cornelissen, Ivo; Casad, Michelle; Roahrig, Julie; Castellino, Francis J.; Rosen, Elliot.

In: Experimental Biology and Medicine, Vol. 230, No. 11, 12.2005, p. 860-864.

Research output: Contribution to journalArticle

Suckow, MA, Zollman, A, Cornelissen, I, Casad, M, Roahrig, J, Castellino, FJ & Rosen, E 2005, 'Tissue distribution of fetal liver cells following in utero transplantation in mice', Experimental Biology and Medicine, vol. 230, no. 11, pp. 860-864.
Suckow MA, Zollman A, Cornelissen I, Casad M, Roahrig J, Castellino FJ et al. Tissue distribution of fetal liver cells following in utero transplantation in mice. Experimental Biology and Medicine. 2005 Dec;230(11):860-864.
Suckow, Mark A. ; Zollman, Amy ; Cornelissen, Ivo ; Casad, Michelle ; Roahrig, Julie ; Castellino, Francis J. ; Rosen, Elliot. / Tissue distribution of fetal liver cells following in utero transplantation in mice. In: Experimental Biology and Medicine. 2005 ; Vol. 230, No. 11. pp. 860-864.
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