Tissue-engineering-based strategies for regenerative endodontics

M. T P Albuquerque, M. C. Valera, M. Nakashima, J. E. Nör, M. C. Bottino

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Stemming from in vitro and in vivo pre-clinical and human models, tissue-engineering-based strategies continue to demonstrate great potential for the regeneration of the pulp-dentin complex, particularly in necrotic, immature permanent teeth. Nanofibrous scaffolds, which closely resemble the native extracellular matrix, have been successfully synthesized by various techniques, including but not limited to electrospinning. A common goal in scaffold synthesis has been the notion of promoting cell guidance through the careful design and use of a collection of biochemical and physical cues capable of governing and stimulating specific events at the cellular and tissue levels. The latest advances in processing technologies allow for the fabrication of scaffolds where selected bioactive molecules can be delivered locally, thus increasing the possibilities for clinical success. Though electrospun scaffolds have not yet been tested in vivo in either human or animal pulpless models in immature permanent teeth, recent studies have highlighted their regenerative potential both from an in vitro and in vivo (i.e., subcutaneous model) standpoint. Possible applications for these bioactive scaffolds continue to evolve, with significant prospects related to the regeneration of both dentin and pulp tissue and, more recently, to root canal disinfection. Nonetheless, no single implantable scaffold can consistently guide the coordinated growth and development of the multiple tissue types involved in the functional regeneration of the pulp-dentin complex. The purpose of this review is to provide a comprehensive perspective on the latest discoveries related to the use of scaffolds and/or stem cells in regenerative endodontics. The authors focused this review on bioactive nanofibrous scaffolds, injectable scaffolds and stem cells, and pre-clinical findings using stem-cell-based strategies. These topics are discussed in detail in an attempt to provide future direction and to shed light on their potential translation to clinical settings.

Original languageEnglish (US)
Pages (from-to)1222-1231
Number of pages10
JournalJournal of Dental Research
Volume93
Issue number12
DOIs
StatePublished - Dec 25 2014
Externally publishedYes

Fingerprint

Endodontics
Dentin
Tissue Engineering
Regeneration
Stem Cells
Tooth
Dental Pulp Cavity
Disinfection
Growth and Development
Cues
Extracellular Matrix
Animal Models
Technology
Injections
In Vitro Techniques

Keywords

  • dental pulp
  • dentin
  • nanofibers
  • regeneration
  • stem cells
  • tissue scaffolds

ASJC Scopus subject areas

  • Dentistry(all)
  • Medicine(all)

Cite this

Albuquerque, M. T. P., Valera, M. C., Nakashima, M., Nör, J. E., & Bottino, M. C. (2014). Tissue-engineering-based strategies for regenerative endodontics. Journal of Dental Research, 93(12), 1222-1231. https://doi.org/10.1177/0022034514549809

Tissue-engineering-based strategies for regenerative endodontics. / Albuquerque, M. T P; Valera, M. C.; Nakashima, M.; Nör, J. E.; Bottino, M. C.

In: Journal of Dental Research, Vol. 93, No. 12, 25.12.2014, p. 1222-1231.

Research output: Contribution to journalArticle

Albuquerque, MTP, Valera, MC, Nakashima, M, Nör, JE & Bottino, MC 2014, 'Tissue-engineering-based strategies for regenerative endodontics', Journal of Dental Research, vol. 93, no. 12, pp. 1222-1231. https://doi.org/10.1177/0022034514549809
Albuquerque MTP, Valera MC, Nakashima M, Nör JE, Bottino MC. Tissue-engineering-based strategies for regenerative endodontics. Journal of Dental Research. 2014 Dec 25;93(12):1222-1231. https://doi.org/10.1177/0022034514549809
Albuquerque, M. T P ; Valera, M. C. ; Nakashima, M. ; Nör, J. E. ; Bottino, M. C. / Tissue-engineering-based strategies for regenerative endodontics. In: Journal of Dental Research. 2014 ; Vol. 93, No. 12. pp. 1222-1231.
@article{9327df3572824af6859e57a14929c3c2,
title = "Tissue-engineering-based strategies for regenerative endodontics",
abstract = "Stemming from in vitro and in vivo pre-clinical and human models, tissue-engineering-based strategies continue to demonstrate great potential for the regeneration of the pulp-dentin complex, particularly in necrotic, immature permanent teeth. Nanofibrous scaffolds, which closely resemble the native extracellular matrix, have been successfully synthesized by various techniques, including but not limited to electrospinning. A common goal in scaffold synthesis has been the notion of promoting cell guidance through the careful design and use of a collection of biochemical and physical cues capable of governing and stimulating specific events at the cellular and tissue levels. The latest advances in processing technologies allow for the fabrication of scaffolds where selected bioactive molecules can be delivered locally, thus increasing the possibilities for clinical success. Though electrospun scaffolds have not yet been tested in vivo in either human or animal pulpless models in immature permanent teeth, recent studies have highlighted their regenerative potential both from an in vitro and in vivo (i.e., subcutaneous model) standpoint. Possible applications for these bioactive scaffolds continue to evolve, with significant prospects related to the regeneration of both dentin and pulp tissue and, more recently, to root canal disinfection. Nonetheless, no single implantable scaffold can consistently guide the coordinated growth and development of the multiple tissue types involved in the functional regeneration of the pulp-dentin complex. The purpose of this review is to provide a comprehensive perspective on the latest discoveries related to the use of scaffolds and/or stem cells in regenerative endodontics. The authors focused this review on bioactive nanofibrous scaffolds, injectable scaffolds and stem cells, and pre-clinical findings using stem-cell-based strategies. These topics are discussed in detail in an attempt to provide future direction and to shed light on their potential translation to clinical settings.",
keywords = "dental pulp, dentin, nanofibers, regeneration, stem cells, tissue scaffolds",
author = "Albuquerque, {M. T P} and Valera, {M. C.} and M. Nakashima and N{\"o}r, {J. E.} and Bottino, {M. C.}",
year = "2014",
month = "12",
day = "25",
doi = "10.1177/0022034514549809",
language = "English (US)",
volume = "93",
pages = "1222--1231",
journal = "Journal of Dental Research",
issn = "0022-0345",
publisher = "SAGE Publications Inc.",
number = "12",

}

TY - JOUR

T1 - Tissue-engineering-based strategies for regenerative endodontics

AU - Albuquerque, M. T P

AU - Valera, M. C.

AU - Nakashima, M.

AU - Nör, J. E.

AU - Bottino, M. C.

PY - 2014/12/25

Y1 - 2014/12/25

N2 - Stemming from in vitro and in vivo pre-clinical and human models, tissue-engineering-based strategies continue to demonstrate great potential for the regeneration of the pulp-dentin complex, particularly in necrotic, immature permanent teeth. Nanofibrous scaffolds, which closely resemble the native extracellular matrix, have been successfully synthesized by various techniques, including but not limited to electrospinning. A common goal in scaffold synthesis has been the notion of promoting cell guidance through the careful design and use of a collection of biochemical and physical cues capable of governing and stimulating specific events at the cellular and tissue levels. The latest advances in processing technologies allow for the fabrication of scaffolds where selected bioactive molecules can be delivered locally, thus increasing the possibilities for clinical success. Though electrospun scaffolds have not yet been tested in vivo in either human or animal pulpless models in immature permanent teeth, recent studies have highlighted their regenerative potential both from an in vitro and in vivo (i.e., subcutaneous model) standpoint. Possible applications for these bioactive scaffolds continue to evolve, with significant prospects related to the regeneration of both dentin and pulp tissue and, more recently, to root canal disinfection. Nonetheless, no single implantable scaffold can consistently guide the coordinated growth and development of the multiple tissue types involved in the functional regeneration of the pulp-dentin complex. The purpose of this review is to provide a comprehensive perspective on the latest discoveries related to the use of scaffolds and/or stem cells in regenerative endodontics. The authors focused this review on bioactive nanofibrous scaffolds, injectable scaffolds and stem cells, and pre-clinical findings using stem-cell-based strategies. These topics are discussed in detail in an attempt to provide future direction and to shed light on their potential translation to clinical settings.

AB - Stemming from in vitro and in vivo pre-clinical and human models, tissue-engineering-based strategies continue to demonstrate great potential for the regeneration of the pulp-dentin complex, particularly in necrotic, immature permanent teeth. Nanofibrous scaffolds, which closely resemble the native extracellular matrix, have been successfully synthesized by various techniques, including but not limited to electrospinning. A common goal in scaffold synthesis has been the notion of promoting cell guidance through the careful design and use of a collection of biochemical and physical cues capable of governing and stimulating specific events at the cellular and tissue levels. The latest advances in processing technologies allow for the fabrication of scaffolds where selected bioactive molecules can be delivered locally, thus increasing the possibilities for clinical success. Though electrospun scaffolds have not yet been tested in vivo in either human or animal pulpless models in immature permanent teeth, recent studies have highlighted their regenerative potential both from an in vitro and in vivo (i.e., subcutaneous model) standpoint. Possible applications for these bioactive scaffolds continue to evolve, with significant prospects related to the regeneration of both dentin and pulp tissue and, more recently, to root canal disinfection. Nonetheless, no single implantable scaffold can consistently guide the coordinated growth and development of the multiple tissue types involved in the functional regeneration of the pulp-dentin complex. The purpose of this review is to provide a comprehensive perspective on the latest discoveries related to the use of scaffolds and/or stem cells in regenerative endodontics. The authors focused this review on bioactive nanofibrous scaffolds, injectable scaffolds and stem cells, and pre-clinical findings using stem-cell-based strategies. These topics are discussed in detail in an attempt to provide future direction and to shed light on their potential translation to clinical settings.

KW - dental pulp

KW - dentin

KW - nanofibers

KW - regeneration

KW - stem cells

KW - tissue scaffolds

UR - http://www.scopus.com/inward/record.url?scp=84911878024&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84911878024&partnerID=8YFLogxK

U2 - 10.1177/0022034514549809

DO - 10.1177/0022034514549809

M3 - Article

C2 - 25201917

AN - SCOPUS:84911878024

VL - 93

SP - 1222

EP - 1231

JO - Journal of Dental Research

JF - Journal of Dental Research

SN - 0022-0345

IS - 12

ER -