Tissue-Resident Macrophages Limit Pulmonary CD8 Resident Memory T Cell Establishment

Nick P. Goplen, Su Huang, Bibo Zhu, In Su Cheon, Young Min Son, Zheng Wang, Chaofan Li, Qigang Dai, Li Jiang, Jie Sun

Research output: Contribution to journalArticle

Abstract

Tissue resident memory CD8 T cells (TRM) serve as potent local sentinels and contribute significantly to protective immunity against intracellular mucosal pathogens. While the molecular and transcriptional underpinnings of TRM differentiation are emerging, how TRM establishment is regulated by other leukocytes in vivo is largely unclear. Here, we observed that expression of PPAR-γ in the myeloid compartment was a negative regulator of CD8 TRM establishment following influenza virus infection. Interestingly, myeloid deficiency of PPAR-γ resulted in selective impairment of the tissue-resident alveolar macrophage (AM) compartment during primary influenza infection, suggesting that AM are likely negative regulators of CD8 TRM differentiation. Indeed, influenza-specific CD8 TRM cell numbers were increased following early, but not late ablation of AM using the CD169-DTR model. Importantly, these findings were specific to the parenchyma of infected tissue as circulating memory T cell frequencies in lung and TCM and TEM in spleen were largely unaltered following macrophage ablation. Further, the magnitude of the effector response could not explain these observations. These data indicate local regulation of pulmonary TRM differentiation is alveolar macrophage dependent. These, findings could aid in vaccine design aimed at increasing TRM density to enhance protective immunity, or deflating their numbers in conditions where they cause overt or veiled chronic pathologies.

Original languageEnglish (US)
Article number2332
JournalFrontiers in immunology
Volume10
DOIs
StatePublished - Oct 10 2019
Externally publishedYes

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Alveolar Macrophages
T-Lymphocytes
Peroxisome Proliferator-Activated Receptors
Human Influenza
Immunity
Lung
Virus Diseases
Orthomyxoviridae
Leukocytes
Vaccines
Spleen
Cell Count
Macrophages
Pathology
Infection

Keywords

  • alveolar macrophage
  • CD169
  • CD69
  • CD8 T cell differentiation
  • influenza
  • PPAR-γ
  • tissue-resident memory

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Tissue-Resident Macrophages Limit Pulmonary CD8 Resident Memory T Cell Establishment. / Goplen, Nick P.; Huang, Su; Zhu, Bibo; Cheon, In Su; Son, Young Min; Wang, Zheng; Li, Chaofan; Dai, Qigang; Jiang, Li; Sun, Jie.

In: Frontiers in immunology, Vol. 10, 2332, 10.10.2019.

Research output: Contribution to journalArticle

Goplen, NP, Huang, S, Zhu, B, Cheon, IS, Son, YM, Wang, Z, Li, C, Dai, Q, Jiang, L & Sun, J 2019, 'Tissue-Resident Macrophages Limit Pulmonary CD8 Resident Memory T Cell Establishment', Frontiers in immunology, vol. 10, 2332. https://doi.org/10.3389/fimmu.2019.02332
Goplen, Nick P. ; Huang, Su ; Zhu, Bibo ; Cheon, In Su ; Son, Young Min ; Wang, Zheng ; Li, Chaofan ; Dai, Qigang ; Jiang, Li ; Sun, Jie. / Tissue-Resident Macrophages Limit Pulmonary CD8 Resident Memory T Cell Establishment. In: Frontiers in immunology. 2019 ; Vol. 10.
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