Tissue specific and developmental expression of rat long-and medium-chain acyl-CoA dehydrogenases

Bryan Hainline, Debra J. Kahlenbeck, James Grant, Arnold W. Strauss

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Utilization of fatty acids for energy varies among mammalian tissues and during development due to changes in expression of enzymes of mitochondrial β oxidation. To discern whether two related nuclear genes are expressed similarly, the tissue distribution and developmental profile of the rat long- and medium-chain acyl-CoA dehydrogenase (LCAD and MCAD) mRNAs were compared. A 1451 base full-length LCAD cDNA from neonatal rat aorta was used to study mRNA accumulation in adult and fetal rat tissues. LCAD and MCAD mRNAs were expressed in aorta, heart, and brown fat at levels 8-40 fold greater than in liver, kidney, and duodenum. Brain, placenta, ovary, testes, and skeletal muscle showed the least mRNA. Western blots of adult tissues with anti-rat LCAD antiserum showed corresponding amounts of LCAD protein subunits. LCAD mRNA was detectable in heart, liver, kidney, and brain of fetal rats and increased with age. LCAD and MCAD mRNAs were present in brown fat in 2-10 fold greater amounts compared to other tissues from the newborn period to the end of the weaning period. The high level of expression of LCAD and MCAD mRNA in aorta, heart, and brown fat likely reflects the high energy requirements of those tissues. Differential expression of LCAD and MCAD mRNAs reflects not only inherent gene prescribed programs, but also external influences such as hormones and diet.

Original languageEnglish
Pages (from-to)460-468
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1216
Issue number3
DOIs
StatePublished - Dec 14 1993

Fingerprint

Long-Chain Acyl-CoA Dehydrogenase
Acyl-CoA Dehydrogenase
Rats
Tissue
Messenger RNA
Brown Adipose Tissue
Aorta
Fats
Liver
Brain
Genes
Kidney
Protein Subunits
Tissue Distribution
Nutrition
Weaning
Duodenum
Placenta
Muscle
Testis

Keywords

  • (Rat)
  • Brown fat
  • Developmental regulation
  • Fatty acid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Tissue specific and developmental expression of rat long-and medium-chain acyl-CoA dehydrogenases. / Hainline, Bryan; Kahlenbeck, Debra J.; Grant, James; Strauss, Arnold W.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1216, No. 3, 14.12.1993, p. 460-468.

Research output: Contribution to journalArticle

@article{7e0eb9ab8c4f4fc4957558284b3fcf72,
title = "Tissue specific and developmental expression of rat long-and medium-chain acyl-CoA dehydrogenases",
abstract = "Utilization of fatty acids for energy varies among mammalian tissues and during development due to changes in expression of enzymes of mitochondrial β oxidation. To discern whether two related nuclear genes are expressed similarly, the tissue distribution and developmental profile of the rat long- and medium-chain acyl-CoA dehydrogenase (LCAD and MCAD) mRNAs were compared. A 1451 base full-length LCAD cDNA from neonatal rat aorta was used to study mRNA accumulation in adult and fetal rat tissues. LCAD and MCAD mRNAs were expressed in aorta, heart, and brown fat at levels 8-40 fold greater than in liver, kidney, and duodenum. Brain, placenta, ovary, testes, and skeletal muscle showed the least mRNA. Western blots of adult tissues with anti-rat LCAD antiserum showed corresponding amounts of LCAD protein subunits. LCAD mRNA was detectable in heart, liver, kidney, and brain of fetal rats and increased with age. LCAD and MCAD mRNAs were present in brown fat in 2-10 fold greater amounts compared to other tissues from the newborn period to the end of the weaning period. The high level of expression of LCAD and MCAD mRNA in aorta, heart, and brown fat likely reflects the high energy requirements of those tissues. Differential expression of LCAD and MCAD mRNAs reflects not only inherent gene prescribed programs, but also external influences such as hormones and diet.",
keywords = "(Rat), Brown fat, Developmental regulation, Fatty acid",
author = "Bryan Hainline and Kahlenbeck, {Debra J.} and James Grant and Strauss, {Arnold W.}",
year = "1993",
month = "12",
day = "14",
doi = "10.1016/0167-4781(93)90015-6",
language = "English",
volume = "1216",
pages = "460--468",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - Tissue specific and developmental expression of rat long-and medium-chain acyl-CoA dehydrogenases

AU - Hainline, Bryan

AU - Kahlenbeck, Debra J.

AU - Grant, James

AU - Strauss, Arnold W.

PY - 1993/12/14

Y1 - 1993/12/14

N2 - Utilization of fatty acids for energy varies among mammalian tissues and during development due to changes in expression of enzymes of mitochondrial β oxidation. To discern whether two related nuclear genes are expressed similarly, the tissue distribution and developmental profile of the rat long- and medium-chain acyl-CoA dehydrogenase (LCAD and MCAD) mRNAs were compared. A 1451 base full-length LCAD cDNA from neonatal rat aorta was used to study mRNA accumulation in adult and fetal rat tissues. LCAD and MCAD mRNAs were expressed in aorta, heart, and brown fat at levels 8-40 fold greater than in liver, kidney, and duodenum. Brain, placenta, ovary, testes, and skeletal muscle showed the least mRNA. Western blots of adult tissues with anti-rat LCAD antiserum showed corresponding amounts of LCAD protein subunits. LCAD mRNA was detectable in heart, liver, kidney, and brain of fetal rats and increased with age. LCAD and MCAD mRNAs were present in brown fat in 2-10 fold greater amounts compared to other tissues from the newborn period to the end of the weaning period. The high level of expression of LCAD and MCAD mRNA in aorta, heart, and brown fat likely reflects the high energy requirements of those tissues. Differential expression of LCAD and MCAD mRNAs reflects not only inherent gene prescribed programs, but also external influences such as hormones and diet.

AB - Utilization of fatty acids for energy varies among mammalian tissues and during development due to changes in expression of enzymes of mitochondrial β oxidation. To discern whether two related nuclear genes are expressed similarly, the tissue distribution and developmental profile of the rat long- and medium-chain acyl-CoA dehydrogenase (LCAD and MCAD) mRNAs were compared. A 1451 base full-length LCAD cDNA from neonatal rat aorta was used to study mRNA accumulation in adult and fetal rat tissues. LCAD and MCAD mRNAs were expressed in aorta, heart, and brown fat at levels 8-40 fold greater than in liver, kidney, and duodenum. Brain, placenta, ovary, testes, and skeletal muscle showed the least mRNA. Western blots of adult tissues with anti-rat LCAD antiserum showed corresponding amounts of LCAD protein subunits. LCAD mRNA was detectable in heart, liver, kidney, and brain of fetal rats and increased with age. LCAD and MCAD mRNAs were present in brown fat in 2-10 fold greater amounts compared to other tissues from the newborn period to the end of the weaning period. The high level of expression of LCAD and MCAD mRNA in aorta, heart, and brown fat likely reflects the high energy requirements of those tissues. Differential expression of LCAD and MCAD mRNAs reflects not only inherent gene prescribed programs, but also external influences such as hormones and diet.

KW - (Rat)

KW - Brown fat

KW - Developmental regulation

KW - Fatty acid

UR - http://www.scopus.com/inward/record.url?scp=0027771771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027771771&partnerID=8YFLogxK

U2 - 10.1016/0167-4781(93)90015-6

DO - 10.1016/0167-4781(93)90015-6

M3 - Article

C2 - 8268228

AN - SCOPUS:0027771771

VL - 1216

SP - 460

EP - 468

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 3

ER -