Tissue transglutaminase and the development of kidney fibrosis

T. S. Johnson, M. Griffin, G. L. Thomas, B. Yang, N. Skill, A. Cox, C. Kubaral, A. M. El Hahas

Research output: Contribution to journalArticle

Abstract

Transglutaminases (Tg) are a family of calcium-dependent enzymes that catalyse the crosslinking of polypeptide chains, including those of extracellular matrix (ECM) proteins, through the formation of e(v-glutamyl) lysine bonds. This leads to the formation of protein polymers that are highly resistant to degradation. Consequently, tissue Tg (tTg) has been associated with ECM protein deposition in fibrotic diseases such as pulmonary fibrosis and atherosclerosis. hi this study, we have investigated the involvement of tTg in the development of kidney fibrosis in adult male Wistar rats submitted to subtotal nephrectomy (SNx). Groups of 4 to 6 rats were sacrified on days 7, 30, 90, and 120 after SNx. As previously described these rats developed progressive glomerulosclerosis and tubulo-interstitial fibrosis. The tissue level of e(y-glutamyi) lysine crosslink (as determined by exhaustive proteolytic digestion followed by cation exchange HPLC) increased from 347 + 94 pmol/mg protein (M.+S.E.M.) in controls to 1324 + 143 pmol/mg 90 days after SNx (p

Original languageEnglish (US)
JournalBiochemical Society Transactions
Volume24
Issue number4
StatePublished - 1996
Externally publishedYes

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Fibrosis
Tissue
Kidney
Rats
Extracellular Matrix Proteins
Lysine
Transglutaminases
Pulmonary Fibrosis
Nephrectomy
Crosslinking
Cations
Wistar Rats
Digestion
Atherosclerosis
Polymers
Proteins
High Pressure Liquid Chromatography
Calcium
Degradation
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Johnson, T. S., Griffin, M., Thomas, G. L., Yang, B., Skill, N., Cox, A., ... El Hahas, A. M. (1996). Tissue transglutaminase and the development of kidney fibrosis. Biochemical Society Transactions, 24(4).

Tissue transglutaminase and the development of kidney fibrosis. / Johnson, T. S.; Griffin, M.; Thomas, G. L.; Yang, B.; Skill, N.; Cox, A.; Kubaral, C.; El Hahas, A. M.

In: Biochemical Society Transactions, Vol. 24, No. 4, 1996.

Research output: Contribution to journalArticle

Johnson, TS, Griffin, M, Thomas, GL, Yang, B, Skill, N, Cox, A, Kubaral, C & El Hahas, AM 1996, 'Tissue transglutaminase and the development of kidney fibrosis', Biochemical Society Transactions, vol. 24, no. 4.
Johnson TS, Griffin M, Thomas GL, Yang B, Skill N, Cox A et al. Tissue transglutaminase and the development of kidney fibrosis. Biochemical Society Transactions. 1996;24(4).
Johnson, T. S. ; Griffin, M. ; Thomas, G. L. ; Yang, B. ; Skill, N. ; Cox, A. ; Kubaral, C. ; El Hahas, A. M. / Tissue transglutaminase and the development of kidney fibrosis. In: Biochemical Society Transactions. 1996 ; Vol. 24, No. 4.
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AU - Thomas, G. L.

AU - Yang, B.

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AU - Cox, A.

AU - Kubaral, C.

AU - El Hahas, A. M.

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