Tissue uptake, subcellular distribution, and metabolism of 14CH3HgCl and CH3203HgCl by rainbow trout, Salmo gairdneri

Kenneth Olson, K. S. Squibb, R. J. Cousins

Research output: Chapter in Book/Report/Conference proceedingChapter

60 Citations (Scopus)

Abstract

Uptake, whole tissue, and subcellular distribution of methylmercury (MeHg) by rainbow trout, Salmo gairdneri, was studied during, and in three 2-wk intervals following, a single 24-h exposure to 14CH3HgCl and CH3203HgCl. Gel permeation chromatography of cytosol fractions was performed on select tissues. Gills contained approximately 10 times as much MeHg as any other tissue after 24-h exposure whereas concentrations were lowest for brain and skeletal muscle. In the 2-wk period following exposure, gill MeHg concentrations decreased to levels comparable with most other tissues; methylmercury in most other tissues increased and then decreased during the subsequent 4-wk period. Methylmercury levels in brain, skeletal muscle, and gonad were highest at 6 wk. During the 6-wk interval following MeHg exposure, the percent of mercury as inorganic mercury (Hg++) increased in gill, kidney, and liver and to a lesser extent in all other tissues except skeletal muscle. Total MeHg was greatest in cytosol fractions and usually made up from 50 to 80% of the total tissue mercury. In liver cytosol a methylmercury binding metallothionein-like species accounted for up to 40% of the total methylmercury bound. Inorganic mercury (Hg++), probably derived from demethylation of methylmercury, was associated with a metallothionein-like protein detected in gill tissue but no more than 6% of the total mercury in the soluble fraction was bound to this protein(s). Mercury binding to metallothionein-like proteins in kidney and splenic fractions was minimal in spite of relatively large amounts of mercury in the cytosol from these tissues. Metallothionein may act as a binding scavenger for methylmercury and inorganic mercury in fish.

Original languageEnglish (US)
Title of host publicationJ. FISH. RES. BOARD CANADA
Pages381-390
Number of pages10
Volume35
Edition4
StatePublished - 1978
Externally publishedYes

Fingerprint

Oncorhynchus mykiss
Mercury
Metallothionein
Cytosol
Skeletal Muscle
Kidney
Proteins
Liver
Brain
Gonads
Tissue Distribution
Gel Chromatography
Fishes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Olson, K., Squibb, K. S., & Cousins, R. J. (1978). Tissue uptake, subcellular distribution, and metabolism of 14CH3HgCl and CH3203HgCl by rainbow trout, Salmo gairdneri. In J. FISH. RES. BOARD CANADA (4 ed., Vol. 35, pp. 381-390)

Tissue uptake, subcellular distribution, and metabolism of 14CH3HgCl and CH3203HgCl by rainbow trout, Salmo gairdneri. / Olson, Kenneth; Squibb, K. S.; Cousins, R. J.

J. FISH. RES. BOARD CANADA. Vol. 35 4. ed. 1978. p. 381-390.

Research output: Chapter in Book/Report/Conference proceedingChapter

Olson, K, Squibb, KS & Cousins, RJ 1978, Tissue uptake, subcellular distribution, and metabolism of 14CH3HgCl and CH3203HgCl by rainbow trout, Salmo gairdneri. in J. FISH. RES. BOARD CANADA. 4 edn, vol. 35, pp. 381-390.
Olson K, Squibb KS, Cousins RJ. Tissue uptake, subcellular distribution, and metabolism of 14CH3HgCl and CH3203HgCl by rainbow trout, Salmo gairdneri. In J. FISH. RES. BOARD CANADA. 4 ed. Vol. 35. 1978. p. 381-390
Olson, Kenneth ; Squibb, K. S. ; Cousins, R. J. / Tissue uptake, subcellular distribution, and metabolism of 14CH3HgCl and CH3203HgCl by rainbow trout, Salmo gairdneri. J. FISH. RES. BOARD CANADA. Vol. 35 4. ed. 1978. pp. 381-390
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