TMPRSS2-ERG gene fusion is rare compared to PTEN deletions in stage T1a prostate cancer

Kurt W. Fisher, Shaobo Zhang, Mingsheng Wang, Rodolfo Montironi, Lisha Wang, Lee A. Baldrige, Jonas Y. Wang, Gregory T. Maclennan, Sean R. Williamson, Antonio Lopez-Beltran, Liang Cheng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

T1a prostate cancers (cancer found incidentally in transurethral resection, <5% of the tissue) are indolent tumors of the transition zone. The overexpression of ERG and the inactivation of PTEN have been shown to be important drivers of carcinogenesis in large series of prostate cancer, but the genetics of transition zone tumors have not been well characterized. We evaluated the status of ERG and PTEN in formalin-fixed paraffin-embedded tissue using immunohistochemical and FISH analysis in 54 T1a transition zone tumors. The protein expression of ERG was determined using a rabbit monoclonal antibody and nuclear staining was scored as positive or negative. The genomic status of ERG was determined using three colored FISH using an ERG-TMPRSS2 tri-color probe set. The protein expression of PTEN was determined using a rabbit monoclonal antibody and cytoplasmic, and nuclear staining was scored as positive or negative. The genomic status of PTEN was determined using dual color FISH with a PTEN probe and a CEP10 probe. We found ERG rearrangement in 2 of 54 tumors (4%), one with protein overexpression by immunohistochemistry. PTEN inactivation was seen in 13 of 54 tumors (24%). Nine of the 13 PTEN alleles were inactivated by hemizygous deletion. No homozygous PTEN deletion was observed. PTEN deletion and ERG rearrangement were mutually exclusive. ERG rearrangement was rare compared to peripheral zone tumors and to PTEN inactivation in T1a transition zone tumors.

Original languageEnglish (US)
JournalMolecular Carcinogenesis
DOIs
StateAccepted/In press - 2016

Fingerprint

Gene Fusion
Prostatic Neoplasms
Neoplasms
Color
Monoclonal Antibodies
PTEN Phosphohydrolase
Staining and Labeling
Rabbits
Paraffin
Formaldehyde
Carcinogenesis
Proteins
Immunohistochemistry
Alleles

Keywords

  • Fluorescence in situ hybridization
  • Insignificant cancer
  • Prostate
  • PTEN loss
  • T1a
  • TMPRSS2-ERG rearrangement
  • Transition zone

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Cancer Research

Cite this

TMPRSS2-ERG gene fusion is rare compared to PTEN deletions in stage T1a prostate cancer. / Fisher, Kurt W.; Zhang, Shaobo; Wang, Mingsheng; Montironi, Rodolfo; Wang, Lisha; Baldrige, Lee A.; Wang, Jonas Y.; Maclennan, Gregory T.; Williamson, Sean R.; Lopez-Beltran, Antonio; Cheng, Liang.

In: Molecular Carcinogenesis, 2016.

Research output: Contribution to journalArticle

Fisher, KW, Zhang, S, Wang, M, Montironi, R, Wang, L, Baldrige, LA, Wang, JY, Maclennan, GT, Williamson, SR, Lopez-Beltran, A & Cheng, L 2016, 'TMPRSS2-ERG gene fusion is rare compared to PTEN deletions in stage T1a prostate cancer', Molecular Carcinogenesis. https://doi.org/10.1002/mc.22535
Fisher, Kurt W. ; Zhang, Shaobo ; Wang, Mingsheng ; Montironi, Rodolfo ; Wang, Lisha ; Baldrige, Lee A. ; Wang, Jonas Y. ; Maclennan, Gregory T. ; Williamson, Sean R. ; Lopez-Beltran, Antonio ; Cheng, Liang. / TMPRSS2-ERG gene fusion is rare compared to PTEN deletions in stage T1a prostate cancer. In: Molecular Carcinogenesis. 2016.
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abstract = "T1a prostate cancers (cancer found incidentally in transurethral resection, <5{\%} of the tissue) are indolent tumors of the transition zone. The overexpression of ERG and the inactivation of PTEN have been shown to be important drivers of carcinogenesis in large series of prostate cancer, but the genetics of transition zone tumors have not been well characterized. We evaluated the status of ERG and PTEN in formalin-fixed paraffin-embedded tissue using immunohistochemical and FISH analysis in 54 T1a transition zone tumors. The protein expression of ERG was determined using a rabbit monoclonal antibody and nuclear staining was scored as positive or negative. The genomic status of ERG was determined using three colored FISH using an ERG-TMPRSS2 tri-color probe set. The protein expression of PTEN was determined using a rabbit monoclonal antibody and cytoplasmic, and nuclear staining was scored as positive or negative. The genomic status of PTEN was determined using dual color FISH with a PTEN probe and a CEP10 probe. We found ERG rearrangement in 2 of 54 tumors (4{\%}), one with protein overexpression by immunohistochemistry. PTEN inactivation was seen in 13 of 54 tumors (24{\%}). Nine of the 13 PTEN alleles were inactivated by hemizygous deletion. No homozygous PTEN deletion was observed. PTEN deletion and ERG rearrangement were mutually exclusive. ERG rearrangement was rare compared to peripheral zone tumors and to PTEN inactivation in T1a transition zone tumors.",
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T1 - TMPRSS2-ERG gene fusion is rare compared to PTEN deletions in stage T1a prostate cancer

AU - Fisher, Kurt W.

AU - Zhang, Shaobo

AU - Wang, Mingsheng

AU - Montironi, Rodolfo

AU - Wang, Lisha

AU - Baldrige, Lee A.

AU - Wang, Jonas Y.

AU - Maclennan, Gregory T.

AU - Williamson, Sean R.

AU - Lopez-Beltran, Antonio

AU - Cheng, Liang

PY - 2016

Y1 - 2016

N2 - T1a prostate cancers (cancer found incidentally in transurethral resection, <5% of the tissue) are indolent tumors of the transition zone. The overexpression of ERG and the inactivation of PTEN have been shown to be important drivers of carcinogenesis in large series of prostate cancer, but the genetics of transition zone tumors have not been well characterized. We evaluated the status of ERG and PTEN in formalin-fixed paraffin-embedded tissue using immunohistochemical and FISH analysis in 54 T1a transition zone tumors. The protein expression of ERG was determined using a rabbit monoclonal antibody and nuclear staining was scored as positive or negative. The genomic status of ERG was determined using three colored FISH using an ERG-TMPRSS2 tri-color probe set. The protein expression of PTEN was determined using a rabbit monoclonal antibody and cytoplasmic, and nuclear staining was scored as positive or negative. The genomic status of PTEN was determined using dual color FISH with a PTEN probe and a CEP10 probe. We found ERG rearrangement in 2 of 54 tumors (4%), one with protein overexpression by immunohistochemistry. PTEN inactivation was seen in 13 of 54 tumors (24%). Nine of the 13 PTEN alleles were inactivated by hemizygous deletion. No homozygous PTEN deletion was observed. PTEN deletion and ERG rearrangement were mutually exclusive. ERG rearrangement was rare compared to peripheral zone tumors and to PTEN inactivation in T1a transition zone tumors.

AB - T1a prostate cancers (cancer found incidentally in transurethral resection, <5% of the tissue) are indolent tumors of the transition zone. The overexpression of ERG and the inactivation of PTEN have been shown to be important drivers of carcinogenesis in large series of prostate cancer, but the genetics of transition zone tumors have not been well characterized. We evaluated the status of ERG and PTEN in formalin-fixed paraffin-embedded tissue using immunohistochemical and FISH analysis in 54 T1a transition zone tumors. The protein expression of ERG was determined using a rabbit monoclonal antibody and nuclear staining was scored as positive or negative. The genomic status of ERG was determined using three colored FISH using an ERG-TMPRSS2 tri-color probe set. The protein expression of PTEN was determined using a rabbit monoclonal antibody and cytoplasmic, and nuclear staining was scored as positive or negative. The genomic status of PTEN was determined using dual color FISH with a PTEN probe and a CEP10 probe. We found ERG rearrangement in 2 of 54 tumors (4%), one with protein overexpression by immunohistochemistry. PTEN inactivation was seen in 13 of 54 tumors (24%). Nine of the 13 PTEN alleles were inactivated by hemizygous deletion. No homozygous PTEN deletion was observed. PTEN deletion and ERG rearrangement were mutually exclusive. ERG rearrangement was rare compared to peripheral zone tumors and to PTEN inactivation in T1a transition zone tumors.

KW - Fluorescence in situ hybridization

KW - Insignificant cancer

KW - Prostate

KW - PTEN loss

KW - T1a

KW - TMPRSS2-ERG rearrangement

KW - Transition zone

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