Toll-like receptor 2 mediates proliferation, survival, NF-κB translocation, and cytokine mRNA expression in LIF-maintained mouse embryonic stem cells

Tammi Taylor, Young June Kim, Xuan Ou, Wilbert Derbigny, Hal E. Broxmeyer

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Toll-like receptor (TLR) activation is important in immune responses and in differentiation of hematopoietic stem cells. We detected mRNA expression of TLRs 1, 2, 3, 5, and 6, but not TLRs 4, 7, 8, and 9 in murine (m)ESC line E14, and noted high cell surface protein expression of TLR2, but not TLR4, for mESC lines R1, CGR8, and E14. ESC lines were cultured in the presence of leukemia inhibitory factor (LIF). Pam3Cys enhanced proliferation and survival of the 3 ESC lines. In contrast, lipopolysaccharide (LPS) decreased proliferation and survival. Pam3Cys and LPS effects on proliferation and survival were blocked by antibody to TLR2, suggesting that effects of both Pam3Cys and LPS on these mESC lines were likely mediated through TLR2. E14 ESC line expressed MyD88. Pam3Cys stimulation of E14 ESCs was associated with induced NF-κB translocation, enhanced phosphorylation of IKK-α/β, and enhanced mRNA, but not protein, expression of tumor necrosis factor-α, interferon-γ, and IL-6. TLR2 activation by Pam3Cys or inhibition by LPS was not associated with changes in morphology or expression of alkaline phosphatase, Oct4, SSEA1, KLF4, or Sox2, markers of undifferentiated mESCs. Our studies identify TLR2 as present and functional in E14, R1, and CGR8 mESC lines.

Original languageEnglish (US)
Pages (from-to)1333-1341
Number of pages9
JournalStem Cells and Development
Volume19
Issue number9
DOIs
StatePublished - Sep 1 2010

ASJC Scopus subject areas

  • Hematology
  • Developmental Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Toll-like receptor 2 mediates proliferation, survival, NF-κB translocation, and cytokine mRNA expression in LIF-maintained mouse embryonic stem cells'. Together they form a unique fingerprint.

  • Cite this