Toothpaste factors related to dentine tubule occlusion and dentine protection against erosion and abrasion

Samira Helena João-Souza, Leticia Oba Sakae, Adrian Lussi, Ana Cecilia Corrêa Aranha, Anderson Hara, Tommy Baumann, Tais Scaramucci, Thiago Saads Carvalho

Research output: Contribution to journalArticle


Objectives: To investigate the effect of toothpastes on dentine surface loss and tubule occlusion, and the association of toothpaste-related factors to each of the outcomes. Materials and methods: One hundred and sixty human dentine specimens were randomly distributed into 10 groups, according to different toothpastes. The specimens were submitted to artificial saliva (60 min), citric acid (3 min), and brushing abrasion (25 s; totalizing 2 min in toothpaste slurries). This was repeated five times and two outcome variables were analyzed: dentine surface loss (dSL; μm) and tubule occlusion by measurement of the total area of open tubules (Area-OT; μm2). Data were analyzed with Kruskal-Wallis and Mann-Whitney tests (α = 0.05); bivariate and multivariate regressions were used to model the association of the chemical (pH, concentration of F, Ca2+, and PO4 3− and presence of Sn2+) and physical (% weight of solid particles, particle size, and wettability) factors of the toothpastes to both outcome variables. Results: Toothpastes caused different degrees of dSL and did not differ in Area-OT. All chemical and physical factors, except the presence of Sn2+, were associated with dSL (p < 0.001). Area-OT was associated only with the presence of Sn2+ (p = 0.033). Conclusion: Greater dSL was associated with lower pH, lower concentration of F, higher concentration of Ca2+ and PO4 3−, greater % weight of solid particles, smaller particle size, and lesser wettability, whereas tubule occlusion was associated with the presence of Sn2+. Clinical relevance: Depending on their chemical and physical composition, toothpastes will cause different degrees of dentine tubule occlusion and dentine surface loss. This could, in turn, modulate dentine hypersensitivity.

Original languageEnglish (US)
JournalClinical Oral Investigations
StateAccepted/In press - Jan 1 2019



  • Anti-erosion
  • Dental abrasion
  • Dental erosion
  • Dentinal tubules
  • Desensitizing
  • Toothpaste

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

João-Souza, S. H., Sakae, L. O., Lussi, A., Aranha, A. C. C., Hara, A., Baumann, T., Scaramucci, T., & Carvalho, T. S. (Accepted/In press). Toothpaste factors related to dentine tubule occlusion and dentine protection against erosion and abrasion. Clinical Oral Investigations.