Topical substance P and corneal epithelial wound closure in the rabbit

Robert E. Kingsley, Carl Marfurt

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Purpose. The authors determined the effect of topically applied substance P (SP) on the rate of corneal epithelial wound closure in the rabbit. Methods. Uniform circular lesions, 6.5 mm in diameter, were made bilaterally in the corneal epithelium of 24 rabbits using N-heptanol. Substance P was applied repeatedly to one eye, and the SP1-7 fragment was applied to the contralateral (control) eye until wound closure was obtained. Three concentrations of peptide solution (5 x 10-5 M, 5 x 10-4 M, and 5 x 10-3 M) were tested in separate groups of eight animals each. An additional eight animals received topical applications (5 x 10-7 M) of the neurokinin- 1 (NK1)-specific SP receptor antagonist CP-99,994-01 or its ineffective enantiomer CP-100,263-01. The mean rates of wound healing for each group of experimental and control eyes were determined by linear regression and analyzed by analysis of variance. Results. There were no statistically significant differences in the mean rates of wound closure (range, 0.083 to 0.106 mm/hour) between experimental- and control-treated corneas for any of the four groups tested. Conclusions. The topical application of SP or its NK1 receptor antagonist has no significant effect on the rate of corneal epithelial wound closure in the rabbit.

Original languageEnglish
Pages (from-to)388-395
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number2
StatePublished - 1997

Fingerprint

Substance P
Neurokinin-1 Receptor Antagonists
Rabbits
Wounds and Injuries
Heptanol
Corneal Epithelium
Wound Healing
Cornea
Linear Models
Analysis of Variance
Regression Analysis
Control Groups
Peptides
3-(2-methoxybenzylamino)-2-phenylpiperidine

Keywords

  • CP-100,263-01
  • CP-99,994-01
  • n-heptanol
  • neurokinin-1 (NK1) receptor
  • substance P fragment

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Topical substance P and corneal epithelial wound closure in the rabbit. / Kingsley, Robert E.; Marfurt, Carl.

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 2, 1997, p. 388-395.

Research output: Contribution to journalArticle

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N2 - Purpose. The authors determined the effect of topically applied substance P (SP) on the rate of corneal epithelial wound closure in the rabbit. Methods. Uniform circular lesions, 6.5 mm in diameter, were made bilaterally in the corneal epithelium of 24 rabbits using N-heptanol. Substance P was applied repeatedly to one eye, and the SP1-7 fragment was applied to the contralateral (control) eye until wound closure was obtained. Three concentrations of peptide solution (5 x 10-5 M, 5 x 10-4 M, and 5 x 10-3 M) were tested in separate groups of eight animals each. An additional eight animals received topical applications (5 x 10-7 M) of the neurokinin- 1 (NK1)-specific SP receptor antagonist CP-99,994-01 or its ineffective enantiomer CP-100,263-01. The mean rates of wound healing for each group of experimental and control eyes were determined by linear regression and analyzed by analysis of variance. Results. There were no statistically significant differences in the mean rates of wound closure (range, 0.083 to 0.106 mm/hour) between experimental- and control-treated corneas for any of the four groups tested. Conclusions. The topical application of SP or its NK1 receptor antagonist has no significant effect on the rate of corneal epithelial wound closure in the rabbit.

AB - Purpose. The authors determined the effect of topically applied substance P (SP) on the rate of corneal epithelial wound closure in the rabbit. Methods. Uniform circular lesions, 6.5 mm in diameter, were made bilaterally in the corneal epithelium of 24 rabbits using N-heptanol. Substance P was applied repeatedly to one eye, and the SP1-7 fragment was applied to the contralateral (control) eye until wound closure was obtained. Three concentrations of peptide solution (5 x 10-5 M, 5 x 10-4 M, and 5 x 10-3 M) were tested in separate groups of eight animals each. An additional eight animals received topical applications (5 x 10-7 M) of the neurokinin- 1 (NK1)-specific SP receptor antagonist CP-99,994-01 or its ineffective enantiomer CP-100,263-01. The mean rates of wound healing for each group of experimental and control eyes were determined by linear regression and analyzed by analysis of variance. Results. There were no statistically significant differences in the mean rates of wound closure (range, 0.083 to 0.106 mm/hour) between experimental- and control-treated corneas for any of the four groups tested. Conclusions. The topical application of SP or its NK1 receptor antagonist has no significant effect on the rate of corneal epithelial wound closure in the rabbit.

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