Topographic localization of a 116,000-dalton protein in cartilage

Rose Fife, G. L. Hook, K. D. Brandt

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A disulfide-bonded >400,000-dalton (>400-kD) protein with 116-kD subunits in hyaline cartilage from several species has recently been described. It constitutes 2-4% of the total noncollagenous protein in 4 M guanidinium chloride extracts of normal articular cartilage and accounts for most of the total noncollagen, nonproteoglycan protein synthesized in short-term organ cultures of canine articular cartilage. In the present study, immunofluorensce techniques were used to examine the topographic distribution of the 116-kD subunit protein in normal cartilage. In specimens of normal adult articular cartilage from several species, the protein was located throughout the matrix. More intense staining was observed at the articular surface than in the remainder of the uncalcified cartilage. In contrast, in fetal cartilage, the protein was uniformly distributed throughout the matrix without a marked increase in surface staining. Normal canine menisci and annulus fibrosus also demonstrated moderate fluorescence after incubation with the antiserum to the 116-kD subunit protein. Normal canine nucleus pulposus, synovium, aorta, and monolayer cultures of canine synovial cells exhibited only weak immunofluorescence after incubation with the antiserum. Therefore, the 116-kD subunit protein appears to be a ubiquitous matrix protein in cartilage.

Original languageEnglish
Pages (from-to)127-133
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume33
Issue number2
StatePublished - 1985

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Cartilage
Canidae
Protein Subunits
Articular Cartilage
Immune Sera
Proteins
Matrilin Proteins
Fetal Proteins
Staining and Labeling
Hyaline Cartilage
Synovial Membrane
Organ Culture Techniques
Guanidine
Disulfides
Fluorescent Antibody Technique
Aorta
Joints
Fluorescence

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Topographic localization of a 116,000-dalton protein in cartilage. / Fife, Rose; Hook, G. L.; Brandt, K. D.

In: Journal of Histochemistry and Cytochemistry, Vol. 33, No. 2, 1985, p. 127-133.

Research output: Contribution to journalArticle

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