Topography of bovine papillomavirus E2 protein on the viral genome during the cell cycle

Suzanne M. Melanson, Elliot Androphy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The multifunctional papillomavirus E2 protein serves important roles in transcriptional activation and genome maintenance and cooperates with the viral E1 helicase for the initiation of viral DNA replication. The bovine papillomavirus genome contains seventeen E2 binding sites, largely concentrated within the long control region, and a single E1 binding site at the origin of viral replication. Using chromatin immunoprecipitation (ChIP) followed by restriction enzyme digestion and PCR, we show that BPV E1 was present only in the region of an active origin of replication and that BPV E2 remained attached to definable segments of the viral genome at specific stages of the cell cycle.

Original languageEnglish (US)
Pages (from-to)258-264
Number of pages7
JournalVirology
Volume393
Issue number2
DOIs
StatePublished - Oct 25 2009
Externally publishedYes

Fingerprint

Replication Origin
Viral Genome
Cell Cycle
Binding Sites
Genome
Chromatin Immunoprecipitation
Viral DNA
DNA Replication
Transcriptional Activation
Digestion
Maintenance
Polymerase Chain Reaction
Enzymes
Proteins
Bovine papillomavirus E2 protein

Keywords

  • Bovine papillomavirus
  • BPV-1 genome
  • Papillomavirus E2 protein
  • RED-ChIP

ASJC Scopus subject areas

  • Virology

Cite this

Topography of bovine papillomavirus E2 protein on the viral genome during the cell cycle. / Melanson, Suzanne M.; Androphy, Elliot.

In: Virology, Vol. 393, No. 2, 25.10.2009, p. 258-264.

Research output: Contribution to journalArticle

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