Topography of neuron loss in the retinal ganglion cell layer in human glaucoma

Y. Lei, N. Garrahan, B. Hermann, M. P. Fautsch, D. H. Johnson, M. R. Hernandez, M. Boulton, J. E. Morgan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aim: To determine if retinal ganglion cell (RGC) loss influences the loss of surrounding RGCs to generate clustered patterns of cell death in human glaucoma. It is hypothesised that retinal ganglion cell loss accelerates the loss of surrounding cells to generate, at a local, cellular scale, clustered patterns of retinal of RGC death. The absence of these interactions would result in a diffuse pattern RGC loss. Method: Six glaucomatous retinas (67-83 years old) and six age-matched control retinas (61-89 years old) were prepared as wholemounts and stained by 4′,6-diamidino-2-phenylindole (DAPI) solution (3 mg/ml in PBS). An area corresponding to central 14° of the visual field was imaged. The nearest-neighbour distribution was determined for cells in both normal and glaucomatous RGCL. Results: Clustered RGC loss in human glaucoma was observed on a background of diffuse loss. The mean nearest-neighbour distance (NND) of the glaucomatous retinas was significantly higher than with controls (p

Original languageEnglish (US)
Pages (from-to)1676-1679
Number of pages4
JournalBritish Journal of Ophthalmology
Volume93
Issue number12
DOIs
StatePublished - Dec 2009
Externally publishedYes

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Retinal Ganglion Cells
Glaucoma
Neurons
Retina
Cell Death
Visual Fields

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Lei, Y., Garrahan, N., Hermann, B., Fautsch, M. P., Johnson, D. H., Hernandez, M. R., ... Morgan, J. E. (2009). Topography of neuron loss in the retinal ganglion cell layer in human glaucoma. British Journal of Ophthalmology, 93(12), 1676-1679. https://doi.org/10.1136/bjo.2009.159210

Topography of neuron loss in the retinal ganglion cell layer in human glaucoma. / Lei, Y.; Garrahan, N.; Hermann, B.; Fautsch, M. P.; Johnson, D. H.; Hernandez, M. R.; Boulton, M.; Morgan, J. E.

In: British Journal of Ophthalmology, Vol. 93, No. 12, 12.2009, p. 1676-1679.

Research output: Contribution to journalArticle

Lei, Y, Garrahan, N, Hermann, B, Fautsch, MP, Johnson, DH, Hernandez, MR, Boulton, M & Morgan, JE 2009, 'Topography of neuron loss in the retinal ganglion cell layer in human glaucoma', British Journal of Ophthalmology, vol. 93, no. 12, pp. 1676-1679. https://doi.org/10.1136/bjo.2009.159210
Lei Y, Garrahan N, Hermann B, Fautsch MP, Johnson DH, Hernandez MR et al. Topography of neuron loss in the retinal ganglion cell layer in human glaucoma. British Journal of Ophthalmology. 2009 Dec;93(12):1676-1679. https://doi.org/10.1136/bjo.2009.159210
Lei, Y. ; Garrahan, N. ; Hermann, B. ; Fautsch, M. P. ; Johnson, D. H. ; Hernandez, M. R. ; Boulton, M. ; Morgan, J. E. / Topography of neuron loss in the retinal ganglion cell layer in human glaucoma. In: British Journal of Ophthalmology. 2009 ; Vol. 93, No. 12. pp. 1676-1679.
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