Total body water measurement in normal and diabetic pregnancy: Evidence for maternal and amniotic fluid equilibrium

Scott Denne, D. Patel, S. C. Kalhan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The H2[18O] tracer dilution method was applied to quantify total body water in 6 normal nonpregnant women, 8 normal pregnant women (gestation 29.3 ± 6.0 weeks, range 21-37 weeks), and 8 insulin-dependent diabetic pregnant subjects (gestation 29.3 ± 6.0 weeks, range 21-37 weeks). Plateau or equilibrium enrichment of 18O in the breath CO2 was achieved at approximately 2 h in nonpregnant normal subjects. In contrast, in pregnant subjects equilibrium plateau was reached later, at approximately 3 h. In order to examine whether the amniotic fluid/fetal compartment had also reached equilibrium with the maternal water compartment, amniotic fluid samples were obtained from 5 women undergoing elective cesarean section, approximately 5 h after the administration of tracer H2[18O]. The 18O enrichment of amniotic fluid was similar to that of the mother. Total body water was similar in normal and diabetic pregnant subjects (40.4 ± 4.4 vs. 40.1 ± 5.8 kg) and represented 55.8 ± 5.4 and 58.7 ± 5.5% of body weight, respectively. These data demonstrate the usefulness of the H2[18O] tracer method to measure total body water in pregnancy and emphasize the importance of an adequate equilibrium period 4-5 h in order to measure the dilution of administered tracer. The measurements by H2[18O] tracer are similar to those previously reported wiht deuterium oxide. Insofar as total body water reflects lean body mass, the data suggest that body compositional changes in normal and diabetic pregnancy are qualitatively similar.

Original languageEnglish (US)
Pages (from-to)284-291
Number of pages8
JournalBiology of the Neonate
Volume57
Issue number5
StatePublished - 1990
Externally publishedYes

Fingerprint

Pregnancy in Diabetics
Body Water
Amniotic Fluid
Mothers
Pregnancy
Deuterium Oxide
Cesarean Section
Pregnant Women
Body Weight
Insulin
Water

Keywords

  • Body composition
  • Diabetes
  • Pregnancy
  • Stable isotopes
  • Total body water

ASJC Scopus subject areas

  • Developmental Biology
  • Pediatrics, Perinatology, and Child Health

Cite this

Total body water measurement in normal and diabetic pregnancy : Evidence for maternal and amniotic fluid equilibrium. / Denne, Scott; Patel, D.; Kalhan, S. C.

In: Biology of the Neonate, Vol. 57, No. 5, 1990, p. 284-291.

Research output: Contribution to journalArticle

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