Toxicity of chemical mixtures: Proteomic analysis of persisting liver and kidney protein alterations induced by repeated exposure of rats to JP-8 jet fuel vapor

Frank Witzmann, Robert L. Carpenter, Glenn D. Ritchie, Cody L. Wilson, Alan F. Nordholm, John Rossi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Male Sprague-Dawley rate were exposed by whole body inhalation to 1000 mg/m3 ± 10% JP-8 jet fuel vapor or room air control conditions for 6 h/day, 5 days/week for six consecutive weeks. Following a rest period of 82 days rats were sacrificed, and liver and kidney tissues examined by proteomic methods for both total protein abundance and protein charge modification. Kidney and lung samples were solubilized and separated via large scale, high resolution two-dimensional electrophoresis (2-DE) and gel patterns scanned, digitized and processed for statistical analysis. Through the use of peptide mass fingerprinting, confirmed by sequence tag analysis, three altered proteins were identified and quantified. Numerical, but not significantly different increases were found in total abundance of lamin A (NCBI Accession No. 1346413) in the liver, and of 10-formyltetrahydrofolate dehydrogenase (10-FTHF DH, 1346044) and glutathione-S-transferase (GST; 2393724) in the kidneys of vapor-exposed subjects. Protein charge modification index (CMI) analysis indicated significant alterations (P <0.001) in expressed lamin A and 10-FTHF DH. These persisting changes in liver and kidney proteins are discussed in terms of possible alterations in the functional capacity of exposed subjects.

Original languageEnglish (US)
Pages (from-to)2138-2147
Number of pages10
JournalElectrophoresis
Volume21
Issue number11
DOIs
StatePublished - 2000

Fingerprint

Liver
Proteomics
Toxicity
Rats
Vapors
Kidney
Lamin Type A
Proteins
Peptide Mapping
Glutathione Transferase
Electrophoresis
Inhalation
Sequence Analysis
Statistical methods
Gels
Air
JP8 aviation fuel
Tissue
Lung
Peptides

Keywords

  • 10-Formyltetrahydrofolate dehydrogenase
  • Glutathione-S- transferase
  • Jet fuel
  • JP-8
  • Kidney proteins
  • Lamin A
  • Liver proteins
  • Mass spectrometry
  • Protein modification index
  • Proteomics
  • Two-dimensional electrophoresis
  • Vapor

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Toxicity of chemical mixtures : Proteomic analysis of persisting liver and kidney protein alterations induced by repeated exposure of rats to JP-8 jet fuel vapor. / Witzmann, Frank; Carpenter, Robert L.; Ritchie, Glenn D.; Wilson, Cody L.; Nordholm, Alan F.; Rossi, John.

In: Electrophoresis, Vol. 21, No. 11, 2000, p. 2138-2147.

Research output: Contribution to journalArticle

Witzmann, Frank ; Carpenter, Robert L. ; Ritchie, Glenn D. ; Wilson, Cody L. ; Nordholm, Alan F. ; Rossi, John. / Toxicity of chemical mixtures : Proteomic analysis of persisting liver and kidney protein alterations induced by repeated exposure of rats to JP-8 jet fuel vapor. In: Electrophoresis. 2000 ; Vol. 21, No. 11. pp. 2138-2147.
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