Toxicity of chemical mixtures: Proteomic analysis of persisting liver and kidney protein alterations induced by repeated exposure of rats to JP-8 jet fuel vapor

Frank A. Witzmann, Robert L. Carpenter, Glenn D. Ritchie, Cody L. Wilson, Alan F. Nordholm, John Rossi

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Male Sprague-Dawley rate were exposed by whole body inhalation to 1000 mg/m3 ± 10% JP-8 jet fuel vapor or room air control conditions for 6 h/day, 5 days/week for six consecutive weeks. Following a rest period of 82 days rats were sacrificed, and liver and kidney tissues examined by proteomic methods for both total protein abundance and protein charge modification. Kidney and lung samples were solubilized and separated via large scale, high resolution two-dimensional electrophoresis (2-DE) and gel patterns scanned, digitized and processed for statistical analysis. Through the use of peptide mass fingerprinting, confirmed by sequence tag analysis, three altered proteins were identified and quantified. Numerical, but not significantly different increases were found in total abundance of lamin A (NCBI Accession No. 1346413) in the liver, and of 10-formyltetrahydrofolate dehydrogenase (10-FTHF DH, 1346044) and glutathione-S-transferase (GST; 2393724) in the kidneys of vapor-exposed subjects. Protein charge modification index (CMI) analysis indicated significant alterations (P < 0.001) in expressed lamin A and 10-FTHF DH. These persisting changes in liver and kidney proteins are discussed in terms of possible alterations in the functional capacity of exposed subjects.

Original languageEnglish (US)
Pages (from-to)2138-2147
Number of pages10
JournalELECTROPHORESIS
Volume21
Issue number11
DOIs
StatePublished - Jan 1 2000

Keywords

  • 10-Formyltetrahydrofolate dehydrogenase
  • Glutathione-S- transferase
  • JP-8
  • Jet fuel
  • Kidney proteins
  • Lamin A
  • Liver proteins
  • Mass spectrometry
  • Protein modification index
  • Proteomics
  • Two-dimensional electrophoresis
  • Vapor

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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