Toxoplasma gondii Sis1-like J-domain protein is a cytosolic chaperone associated to HSP90/HSP70 complex

Maria J. Figueras, Osvaldo A. Martin, Pablo C. Echeverria, Natalia de Miguel, Arunasalam Naguleswaran, William Sullivan, Maria M. Corvi, Sergio O. Angel

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Toxoplasma gondii is an obligate intracellular protozoan parasite in which 36 predicted Hsp40 family members were identified by searching the T. gondii genome. The predicted protein sequence from the gene ID TGME49_065310 showed an amino acid sequence and domain structure similar to Saccharomyces cerevisiae Sis1. TgSis1 did not show differences in its expression profile during alkaline stress by microarray analysis. Furthermore, TgSis1 showed to be a cytosolic Hsp40 which co-immunoprecipitated with T. gondii Hsp70 and Hsp90. Structural modeling of the TgSis1 peptide binding fragment revealed structural and electrostatic properties different from the experimental model of human Sis1-like protein (Hdj1). Based on these differences; we propose that TgSis1 may be a potentially attractive drug target for developing a novel anti- T. gondii therapy.

Original languageEnglish
Pages (from-to)725-733
Number of pages9
JournalInternational Journal of Biological Macromolecules
Volume50
Issue number3
DOIs
StatePublished - Apr 1 2012

Fingerprint

Toxoplasma
Genes
Microarrays
Yeast
Electrostatics
Peptide Fragments
Proteins
Microarray Analysis
Static Electricity
Amino Acids
Peptides
Saccharomyces cerevisiae
Amino Acid Sequence
Parasites
Theoretical Models
Pharmaceutical Preparations
Genome
Protein Domains
Therapeutics
Thomsen-Friedenreich antibodies

Keywords

  • HSP40
  • HSP90
  • Toxoplasma

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Cite this

Toxoplasma gondii Sis1-like J-domain protein is a cytosolic chaperone associated to HSP90/HSP70 complex. / Figueras, Maria J.; Martin, Osvaldo A.; Echeverria, Pablo C.; de Miguel, Natalia; Naguleswaran, Arunasalam; Sullivan, William; Corvi, Maria M.; Angel, Sergio O.

In: International Journal of Biological Macromolecules, Vol. 50, No. 3, 01.04.2012, p. 725-733.

Research output: Contribution to journalArticle

Figueras, Maria J. ; Martin, Osvaldo A. ; Echeverria, Pablo C. ; de Miguel, Natalia ; Naguleswaran, Arunasalam ; Sullivan, William ; Corvi, Maria M. ; Angel, Sergio O. / Toxoplasma gondii Sis1-like J-domain protein is a cytosolic chaperone associated to HSP90/HSP70 complex. In: International Journal of Biological Macromolecules. 2012 ; Vol. 50, No. 3. pp. 725-733.
@article{e51c1ff19f114c519a8de18944f8fdf6,
title = "Toxoplasma gondii Sis1-like J-domain protein is a cytosolic chaperone associated to HSP90/HSP70 complex",
abstract = "Toxoplasma gondii is an obligate intracellular protozoan parasite in which 36 predicted Hsp40 family members were identified by searching the T. gondii genome. The predicted protein sequence from the gene ID TGME49_065310 showed an amino acid sequence and domain structure similar to Saccharomyces cerevisiae Sis1. TgSis1 did not show differences in its expression profile during alkaline stress by microarray analysis. Furthermore, TgSis1 showed to be a cytosolic Hsp40 which co-immunoprecipitated with T. gondii Hsp70 and Hsp90. Structural modeling of the TgSis1 peptide binding fragment revealed structural and electrostatic properties different from the experimental model of human Sis1-like protein (Hdj1). Based on these differences; we propose that TgSis1 may be a potentially attractive drug target for developing a novel anti- T. gondii therapy.",
keywords = "HSP40, HSP90, Toxoplasma",
author = "Figueras, {Maria J.} and Martin, {Osvaldo A.} and Echeverria, {Pablo C.} and {de Miguel}, Natalia and Arunasalam Naguleswaran and William Sullivan and Corvi, {Maria M.} and Angel, {Sergio O.}",
year = "2012",
month = "4",
day = "1",
doi = "10.1016/j.ijbiomac.2011.12.012",
language = "English",
volume = "50",
pages = "725--733",
journal = "International Journal of Biological Macromolecules",
issn = "0141-8130",
publisher = "Elsevier",
number = "3",

}

TY - JOUR

T1 - Toxoplasma gondii Sis1-like J-domain protein is a cytosolic chaperone associated to HSP90/HSP70 complex

AU - Figueras, Maria J.

AU - Martin, Osvaldo A.

AU - Echeverria, Pablo C.

AU - de Miguel, Natalia

AU - Naguleswaran, Arunasalam

AU - Sullivan, William

AU - Corvi, Maria M.

AU - Angel, Sergio O.

PY - 2012/4/1

Y1 - 2012/4/1

N2 - Toxoplasma gondii is an obligate intracellular protozoan parasite in which 36 predicted Hsp40 family members were identified by searching the T. gondii genome. The predicted protein sequence from the gene ID TGME49_065310 showed an amino acid sequence and domain structure similar to Saccharomyces cerevisiae Sis1. TgSis1 did not show differences in its expression profile during alkaline stress by microarray analysis. Furthermore, TgSis1 showed to be a cytosolic Hsp40 which co-immunoprecipitated with T. gondii Hsp70 and Hsp90. Structural modeling of the TgSis1 peptide binding fragment revealed structural and electrostatic properties different from the experimental model of human Sis1-like protein (Hdj1). Based on these differences; we propose that TgSis1 may be a potentially attractive drug target for developing a novel anti- T. gondii therapy.

AB - Toxoplasma gondii is an obligate intracellular protozoan parasite in which 36 predicted Hsp40 family members were identified by searching the T. gondii genome. The predicted protein sequence from the gene ID TGME49_065310 showed an amino acid sequence and domain structure similar to Saccharomyces cerevisiae Sis1. TgSis1 did not show differences in its expression profile during alkaline stress by microarray analysis. Furthermore, TgSis1 showed to be a cytosolic Hsp40 which co-immunoprecipitated with T. gondii Hsp70 and Hsp90. Structural modeling of the TgSis1 peptide binding fragment revealed structural and electrostatic properties different from the experimental model of human Sis1-like protein (Hdj1). Based on these differences; we propose that TgSis1 may be a potentially attractive drug target for developing a novel anti- T. gondii therapy.

KW - HSP40

KW - HSP90

KW - Toxoplasma

UR - http://www.scopus.com/inward/record.url?scp=84857782228&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857782228&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2011.12.012

DO - 10.1016/j.ijbiomac.2011.12.012

M3 - Article

VL - 50

SP - 725

EP - 733

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

IS - 3

ER -