Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi

Tricia L. Humphreys, Lee Ann Baldridge, Steven D. Billings, James J. Campbell, Stanley Spinola

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

T-cell homing to infected skin is not well studied in humans. We examined sites experimentally infected with Haemophilus ducreyi by immunohistochemistry and flow cytometry for expression of receptors and ligands involved in cutaneous T-cell homing and determined the phenotypes of the T cells that trafficked to skin. Endothelial cells expressed E-selectin in infected but not uninfected skin, while peripheral node addressin (PNAd) was minimally expressed in all samples. CC chemokine ligand 27 (CCL27) was expressed in the epidermis and endothelium of both infected and uninfected skin. Interestingly, CCL21, a chemokine thought to be associated principally with T-cell trafficking in the lymphatic compartment, was highly expressed on the endothelium of infected skin. Few naive cells were present in experimental lesions, emphasizing the combined role of PNAd and CCL21 in trafficking of this subset. Memory cells (CD45RA -) dominated both CD4 and CD8 T-cell populations at the site of infection. Effector memory (CD45RA- CD27-) CD4+ and CD8+ T cells were enriched in lesions. Although the CC chemokine receptor 7-positive (CCR7+) population of both central memory (CD45RA- CD27+) and effector memory cells was not enriched in the skin compared to peripheral blood, CCR71 cells were not precluded from entering infected skin. Taken together with our previous work (D. Soler, T. L. Humphreys, S. M. Spinola, and J. J. Campbell, Blood 101:1677-1683, 2003), these studies led us to propose a model of memory T-cell trafficking to skin in response to experimental H. ducreyi infection.

Original languageEnglish
Pages (from-to)3896-3902
Number of pages7
JournalInfection and Immunity
Volume73
Issue number7
DOIs
StatePublished - Jul 2005

Fingerprint

Haemophilus ducreyi
Skin
T-Lymphocytes
Endothelium
CCR7 Receptors
Chemokine CCL21
Haemophilus Infections
Ligands
CC Chemokines
E-Selectin
Epidermis
Population
Blood Cells
Flow Cytometry
Endothelial Cells
Immunohistochemistry

ASJC Scopus subject areas

  • Immunology

Cite this

Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi. / Humphreys, Tricia L.; Baldridge, Lee Ann; Billings, Steven D.; Campbell, James J.; Spinola, Stanley.

In: Infection and Immunity, Vol. 73, No. 7, 07.2005, p. 3896-3902.

Research output: Contribution to journalArticle

Humphreys, Tricia L. ; Baldridge, Lee Ann ; Billings, Steven D. ; Campbell, James J. ; Spinola, Stanley. / Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi. In: Infection and Immunity. 2005 ; Vol. 73, No. 7. pp. 3896-3902.
@article{ea05945f47c244db81bcf3e542756316,
title = "Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi",
abstract = "T-cell homing to infected skin is not well studied in humans. We examined sites experimentally infected with Haemophilus ducreyi by immunohistochemistry and flow cytometry for expression of receptors and ligands involved in cutaneous T-cell homing and determined the phenotypes of the T cells that trafficked to skin. Endothelial cells expressed E-selectin in infected but not uninfected skin, while peripheral node addressin (PNAd) was minimally expressed in all samples. CC chemokine ligand 27 (CCL27) was expressed in the epidermis and endothelium of both infected and uninfected skin. Interestingly, CCL21, a chemokine thought to be associated principally with T-cell trafficking in the lymphatic compartment, was highly expressed on the endothelium of infected skin. Few naive cells were present in experimental lesions, emphasizing the combined role of PNAd and CCL21 in trafficking of this subset. Memory cells (CD45RA -) dominated both CD4 and CD8 T-cell populations at the site of infection. Effector memory (CD45RA- CD27-) CD4+ and CD8+ T cells were enriched in lesions. Although the CC chemokine receptor 7-positive (CCR7+) population of both central memory (CD45RA- CD27+) and effector memory cells was not enriched in the skin compared to peripheral blood, CCR71 cells were not precluded from entering infected skin. Taken together with our previous work (D. Soler, T. L. Humphreys, S. M. Spinola, and J. J. Campbell, Blood 101:1677-1683, 2003), these studies led us to propose a model of memory T-cell trafficking to skin in response to experimental H. ducreyi infection.",
author = "Humphreys, {Tricia L.} and Baldridge, {Lee Ann} and Billings, {Steven D.} and Campbell, {James J.} and Stanley Spinola",
year = "2005",
month = "7",
doi = "10.1128/IAI.73.7.3896-3902.2005",
language = "English",
volume = "73",
pages = "3896--3902",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "7",

}

TY - JOUR

T1 - Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi

AU - Humphreys, Tricia L.

AU - Baldridge, Lee Ann

AU - Billings, Steven D.

AU - Campbell, James J.

AU - Spinola, Stanley

PY - 2005/7

Y1 - 2005/7

N2 - T-cell homing to infected skin is not well studied in humans. We examined sites experimentally infected with Haemophilus ducreyi by immunohistochemistry and flow cytometry for expression of receptors and ligands involved in cutaneous T-cell homing and determined the phenotypes of the T cells that trafficked to skin. Endothelial cells expressed E-selectin in infected but not uninfected skin, while peripheral node addressin (PNAd) was minimally expressed in all samples. CC chemokine ligand 27 (CCL27) was expressed in the epidermis and endothelium of both infected and uninfected skin. Interestingly, CCL21, a chemokine thought to be associated principally with T-cell trafficking in the lymphatic compartment, was highly expressed on the endothelium of infected skin. Few naive cells were present in experimental lesions, emphasizing the combined role of PNAd and CCL21 in trafficking of this subset. Memory cells (CD45RA -) dominated both CD4 and CD8 T-cell populations at the site of infection. Effector memory (CD45RA- CD27-) CD4+ and CD8+ T cells were enriched in lesions. Although the CC chemokine receptor 7-positive (CCR7+) population of both central memory (CD45RA- CD27+) and effector memory cells was not enriched in the skin compared to peripheral blood, CCR71 cells were not precluded from entering infected skin. Taken together with our previous work (D. Soler, T. L. Humphreys, S. M. Spinola, and J. J. Campbell, Blood 101:1677-1683, 2003), these studies led us to propose a model of memory T-cell trafficking to skin in response to experimental H. ducreyi infection.

AB - T-cell homing to infected skin is not well studied in humans. We examined sites experimentally infected with Haemophilus ducreyi by immunohistochemistry and flow cytometry for expression of receptors and ligands involved in cutaneous T-cell homing and determined the phenotypes of the T cells that trafficked to skin. Endothelial cells expressed E-selectin in infected but not uninfected skin, while peripheral node addressin (PNAd) was minimally expressed in all samples. CC chemokine ligand 27 (CCL27) was expressed in the epidermis and endothelium of both infected and uninfected skin. Interestingly, CCL21, a chemokine thought to be associated principally with T-cell trafficking in the lymphatic compartment, was highly expressed on the endothelium of infected skin. Few naive cells were present in experimental lesions, emphasizing the combined role of PNAd and CCL21 in trafficking of this subset. Memory cells (CD45RA -) dominated both CD4 and CD8 T-cell populations at the site of infection. Effector memory (CD45RA- CD27-) CD4+ and CD8+ T cells were enriched in lesions. Although the CC chemokine receptor 7-positive (CCR7+) population of both central memory (CD45RA- CD27+) and effector memory cells was not enriched in the skin compared to peripheral blood, CCR71 cells were not precluded from entering infected skin. Taken together with our previous work (D. Soler, T. L. Humphreys, S. M. Spinola, and J. J. Campbell, Blood 101:1677-1683, 2003), these studies led us to propose a model of memory T-cell trafficking to skin in response to experimental H. ducreyi infection.

UR - http://www.scopus.com/inward/record.url?scp=21544438762&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21544438762&partnerID=8YFLogxK

U2 - 10.1128/IAI.73.7.3896-3902.2005

DO - 10.1128/IAI.73.7.3896-3902.2005

M3 - Article

C2 - 15972475

AN - SCOPUS:21544438762

VL - 73

SP - 3896

EP - 3902

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 7

ER -