Trans and cis factors affecting A-to-I RNA editing efficiency of a noncoding editing target in C. elegans

Michael C. Washburn, Heather Hundley

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Adenosine-to-inosine RNA editing by ADARs affects thousands of adenosines in an organism's transcriptome. However, adenosines are not edited at equal levels nor do these editing levels correlate well with ADAR expression levels. Therefore, additional mechanisms are utilized by the cell to dictate the editing efficiency at a given adenosine. To examine cis- and transacting factors that regulate A-to-I editing levels specifically in neural cells, we utilized the model organism Caenorhabditis elegans. We demonstrate that a double-stranded RNA (dsRNA) binding protein, ADR-1, inhibits editing in neurons, which is largely masked when examining editing levels from whole animals. Furthermore, expression of ADR-1 and mRNA expression of the editing target can act synergistically to regulate editing efficiency. In addition, we identify a dsRNA region within the Y75B8A.8 3′ UTR that acts as a cis-regulatory element by enhancing ADR-2 editing efficiency. Together, this work identifies mechanisms that regulate editing efficiency of noncoding A-to-I editing sites, which comprise the largest class of ADAR targets.

Original languageEnglish (US)
Pages (from-to)722-728
Number of pages7
JournalRNA
Volume22
Issue number5
DOIs
StatePublished - May 1 2016

Fingerprint

RNA Editing
Adenosine
Inosine
RNA-Binding Proteins
Double-Stranded RNA
Caenorhabditis elegans
3' Untranslated Regions
Transcriptome
Neurons
Messenger RNA
immune RNA

Keywords

  • ADAR
  • Double-stranded RNA
  • Neurons
  • Regulation of RNA editing
  • RNA editing

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Trans and cis factors affecting A-to-I RNA editing efficiency of a noncoding editing target in C. elegans. / Washburn, Michael C.; Hundley, Heather.

In: RNA, Vol. 22, No. 5, 01.05.2016, p. 722-728.

Research output: Contribution to journalArticle

Washburn, Michael C. ; Hundley, Heather. / Trans and cis factors affecting A-to-I RNA editing efficiency of a noncoding editing target in C. elegans. In: RNA. 2016 ; Vol. 22, No. 5. pp. 722-728.
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