Transcription factors C/EBP-β and -δ regulate IL-6 production in IL-1β-stimulated human enterocytes

Eric S. Hungness, Guang Ju Luo, Timothy A. Pritts, Xiaoyan Sun, Bruce Robb, Dan Hershko, Per Olof Hasselgren

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

In recent studies, treatment with IL-1β of cultured Caco-2 cells, a human intestinal epithelial cell line, resulted in transcriptional upregulation of IL-6 production. The role of C/EBP-β and -δ in enterocyte IL-6 production is not known. Stimulation with IL-1β of Caco-2 cells transiently transfected with a luciferase reporter plasmid containing a wild-type IL-6 promoter resulted in an approximately 3.5-fold increase in luciferase activity. This effect of IL-1β was reduced by approximately 30% when the C/EBP binding site in the IL-6 promoter was mutated, supporting a role of C/EBP in the regulation of IL-6 production. When Caco-2 cells were treated with IL-1β in the presence of the MAPK inhibitor, PD-98059, IL-6 mRNA and protein levels were reduced by the same concentrations of PD-98059 that inhibited C/EBP DNA binding activity in previous studies. Finally, overexpression of C/EBP-β and -δ in IL-1β-treated Caco-2 cells resulted in a 10-12-fold increase in IL-6 production. The results suggest that the β and δ isoforms of the C/EBP family of transcription factors at least in part regulate IL-6 production in human intestinal epithelial cells.

Original languageEnglish (US)
Pages (from-to)64-70
Number of pages7
JournalJournal of Cellular Physiology
Volume192
Issue number1
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Enterocytes
Interleukin-1
Interleukin-6
Transcription Factors
Caco-2 Cells
Luciferases
Epithelial Cells
Cultured Cells
Protein Isoforms
Plasmids
Up-Regulation
Binding Sites
Cell Line
Messenger RNA
DNA

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Transcription factors C/EBP-β and -δ regulate IL-6 production in IL-1β-stimulated human enterocytes. / Hungness, Eric S.; Luo, Guang Ju; Pritts, Timothy A.; Sun, Xiaoyan; Robb, Bruce; Hershko, Dan; Hasselgren, Per Olof.

In: Journal of Cellular Physiology, Vol. 192, No. 1, 2002, p. 64-70.

Research output: Contribution to journalArticle

Hungness, Eric S. ; Luo, Guang Ju ; Pritts, Timothy A. ; Sun, Xiaoyan ; Robb, Bruce ; Hershko, Dan ; Hasselgren, Per Olof. / Transcription factors C/EBP-β and -δ regulate IL-6 production in IL-1β-stimulated human enterocytes. In: Journal of Cellular Physiology. 2002 ; Vol. 192, No. 1. pp. 64-70.
@article{aceb9d7100884e5ca47a4445aefa6569,
title = "Transcription factors C/EBP-β and -δ regulate IL-6 production in IL-1β-stimulated human enterocytes",
abstract = "In recent studies, treatment with IL-1β of cultured Caco-2 cells, a human intestinal epithelial cell line, resulted in transcriptional upregulation of IL-6 production. The role of C/EBP-β and -δ in enterocyte IL-6 production is not known. Stimulation with IL-1β of Caco-2 cells transiently transfected with a luciferase reporter plasmid containing a wild-type IL-6 promoter resulted in an approximately 3.5-fold increase in luciferase activity. This effect of IL-1β was reduced by approximately 30{\%} when the C/EBP binding site in the IL-6 promoter was mutated, supporting a role of C/EBP in the regulation of IL-6 production. When Caco-2 cells were treated with IL-1β in the presence of the MAPK inhibitor, PD-98059, IL-6 mRNA and protein levels were reduced by the same concentrations of PD-98059 that inhibited C/EBP DNA binding activity in previous studies. Finally, overexpression of C/EBP-β and -δ in IL-1β-treated Caco-2 cells resulted in a 10-12-fold increase in IL-6 production. The results suggest that the β and δ isoforms of the C/EBP family of transcription factors at least in part regulate IL-6 production in human intestinal epithelial cells.",
author = "Hungness, {Eric S.} and Luo, {Guang Ju} and Pritts, {Timothy A.} and Xiaoyan Sun and Bruce Robb and Dan Hershko and Hasselgren, {Per Olof}",
year = "2002",
doi = "10.1002/jcp.10116",
language = "English (US)",
volume = "192",
pages = "64--70",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Transcription factors C/EBP-β and -δ regulate IL-6 production in IL-1β-stimulated human enterocytes

AU - Hungness, Eric S.

AU - Luo, Guang Ju

AU - Pritts, Timothy A.

AU - Sun, Xiaoyan

AU - Robb, Bruce

AU - Hershko, Dan

AU - Hasselgren, Per Olof

PY - 2002

Y1 - 2002

N2 - In recent studies, treatment with IL-1β of cultured Caco-2 cells, a human intestinal epithelial cell line, resulted in transcriptional upregulation of IL-6 production. The role of C/EBP-β and -δ in enterocyte IL-6 production is not known. Stimulation with IL-1β of Caco-2 cells transiently transfected with a luciferase reporter plasmid containing a wild-type IL-6 promoter resulted in an approximately 3.5-fold increase in luciferase activity. This effect of IL-1β was reduced by approximately 30% when the C/EBP binding site in the IL-6 promoter was mutated, supporting a role of C/EBP in the regulation of IL-6 production. When Caco-2 cells were treated with IL-1β in the presence of the MAPK inhibitor, PD-98059, IL-6 mRNA and protein levels were reduced by the same concentrations of PD-98059 that inhibited C/EBP DNA binding activity in previous studies. Finally, overexpression of C/EBP-β and -δ in IL-1β-treated Caco-2 cells resulted in a 10-12-fold increase in IL-6 production. The results suggest that the β and δ isoforms of the C/EBP family of transcription factors at least in part regulate IL-6 production in human intestinal epithelial cells.

AB - In recent studies, treatment with IL-1β of cultured Caco-2 cells, a human intestinal epithelial cell line, resulted in transcriptional upregulation of IL-6 production. The role of C/EBP-β and -δ in enterocyte IL-6 production is not known. Stimulation with IL-1β of Caco-2 cells transiently transfected with a luciferase reporter plasmid containing a wild-type IL-6 promoter resulted in an approximately 3.5-fold increase in luciferase activity. This effect of IL-1β was reduced by approximately 30% when the C/EBP binding site in the IL-6 promoter was mutated, supporting a role of C/EBP in the regulation of IL-6 production. When Caco-2 cells were treated with IL-1β in the presence of the MAPK inhibitor, PD-98059, IL-6 mRNA and protein levels were reduced by the same concentrations of PD-98059 that inhibited C/EBP DNA binding activity in previous studies. Finally, overexpression of C/EBP-β and -δ in IL-1β-treated Caco-2 cells resulted in a 10-12-fold increase in IL-6 production. The results suggest that the β and δ isoforms of the C/EBP family of transcription factors at least in part regulate IL-6 production in human intestinal epithelial cells.

UR - http://www.scopus.com/inward/record.url?scp=0036260699&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036260699&partnerID=8YFLogxK

U2 - 10.1002/jcp.10116

DO - 10.1002/jcp.10116

M3 - Article

VL - 192

SP - 64

EP - 70

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 1

ER -