Transcription releases protein VII from adenovirus chromatin

Jiangning Chen, Nuria Morral, Daniel A. Engel

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Adenovirus protein VII is the major protein component of the viral nucleoprotein core. It is a nonspecific DNA-binding protein that condenses viral DNA inside the capsid. Protein VII remains associated with viral chromatin throughout early phase, indicating its continuing role during infection. Here we characterize the release of protein VII from infectious genomes during a time period that corresponds to the late phase of infection. Interestingly, the early viral transactivator E1A, but not other early gene products, is responsible for releasing protein VII by a mechanism that requires ongoing transcription but not viral DNA replication. Moreover transcription per se, in the absence of E1A, is also sufficient to trigger release. Accordingly, a recombinant genome containing only non-coding "stuffer" DNA is unable to support release of protein VII. Our data support a model in which early gene transcription results in a change in the structure of the viral chromatin.

Original languageEnglish
Pages (from-to)411-422
Number of pages12
JournalVirology
Volume369
Issue number2
DOIs
StatePublished - Dec 20 2007

Fingerprint

Adenoviridae
Chromatin
Viral Structures
Viral DNA
Proteins
Genetic Transcription
Genome
Nucleoproteins
Trans-Activators
Capsid
DNA-Binding Proteins
Viral Proteins
Infection
DNA Replication
DNA
Genes

Keywords

  • Adenovirus
  • Chromatin
  • Gene therapy
  • Protein VII
  • Transcription

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Transcription releases protein VII from adenovirus chromatin. / Chen, Jiangning; Morral, Nuria; Engel, Daniel A.

In: Virology, Vol. 369, No. 2, 20.12.2007, p. 411-422.

Research output: Contribution to journalArticle

Chen, Jiangning ; Morral, Nuria ; Engel, Daniel A. / Transcription releases protein VII from adenovirus chromatin. In: Virology. 2007 ; Vol. 369, No. 2. pp. 411-422.
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