Transcriptional activation by the papillomavirus E6 zinc finger oncoprotein

Carmela Lamberti, Lloyd C. Morrissey, Steven R. Grossman, Elliot Androphy

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The introduction of the bovine (BPV) or human papillomavirus E6 gene into susceptible cells can result in their transformation, but there are few clues to the mechanism of action of the E6 gene. The characteristic features of E6 proteins are their small size ( ∼ 150 amino acids) and the potential to form two large zinc fingers. To determine if E6 can function as a transcription factor, the BPV E6 gene was fused to the sequence specific DNA binding peptide encoded by the BPV E2 gene. This chimeric E6-E2 protein trans-activated promoters that incorporated E2 binding elements in both rodent cells and Saccharomyces cerevisiae. In the absence of E6-E2 localization to the target promoter, frans-activation did not occur. Alteration of the cysteine residues at the base of each finger abrogated the transcriptional activity of the E6-E2 hybrids. These data demonstrated that the BPV E6 gene encodes a transcription activation domain and imply that a specific structure of the protein, most likely the zinc fingers, is critical for this function. Since these cysteine mutants are also transformation defective, E6 transcriptional functions may be required for its oncogenic activity.

Original languageEnglish (US)
Pages (from-to)1907-1913
Number of pages7
JournalEMBO Journal
Volume9
Issue number6
StatePublished - 1990
Externally publishedYes

Fingerprint

Oncogene Proteins
Zinc Fingers
Transcriptional Activation
Zinc
Genes
Chemical activation
Cysteine
Proteins
Transcription
Yeast
Fingers
Saccharomyces cerevisiae
Rodentia
Transcription Factors
Amino Acids
Peptides
DNA

Keywords

  • E6 proteins
  • Papillornavirus
  • Transcription
  • Transfortnation
  • Zinc finger

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Lamberti, C., Morrissey, L. C., Grossman, S. R., & Androphy, E. (1990). Transcriptional activation by the papillomavirus E6 zinc finger oncoprotein. EMBO Journal, 9(6), 1907-1913.

Transcriptional activation by the papillomavirus E6 zinc finger oncoprotein. / Lamberti, Carmela; Morrissey, Lloyd C.; Grossman, Steven R.; Androphy, Elliot.

In: EMBO Journal, Vol. 9, No. 6, 1990, p. 1907-1913.

Research output: Contribution to journalArticle

Lamberti, C, Morrissey, LC, Grossman, SR & Androphy, E 1990, 'Transcriptional activation by the papillomavirus E6 zinc finger oncoprotein', EMBO Journal, vol. 9, no. 6, pp. 1907-1913.
Lamberti, Carmela ; Morrissey, Lloyd C. ; Grossman, Steven R. ; Androphy, Elliot. / Transcriptional activation by the papillomavirus E6 zinc finger oncoprotein. In: EMBO Journal. 1990 ; Vol. 9, No. 6. pp. 1907-1913.
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