Transcriptional Events during the Recovery from MRSA Lung Infection

A Mouse Pneumonia Model

Jiwang Chen, Gang Feng, Qiang Guo, Juliane B. Wardenburg, Simon Lin, Ichiro Inoshima, Ryan Deaton, Jason X.J. Yuan, Joe G.N. Garcia, Roberto Machado, Michael Otto, Richard G. Wunderink

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Community associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emerging threat to human health throughout the world. Rodent MRSA pneumonia models mainly focus on the early innate immune responses to MRSA lung infection. However, the molecular pattern and mechanisms of recovery from MRSA lung infection are largely unknown. In this study, a sublethal mouse MRSA pneumonia model was employed to investigate late events during the recovery from MRSA lung infection. We compared lung bacterial clearance, bronchoalveolar lavage fluid (BALF) characterization, lung histology, lung cell proliferation, lung vascular permeability and lung gene expression profiling between days 1 and 3 post MRSA lung infection. Compared to day 1 post infection, bacterial colony counts, BALF total cell number and BALF protein concentration significantly decreased at day 3 post infection. Lung cDNA microarray analysis identified 47 significantly up-regulated and 35 down-regulated genes (p<0.01, 1.5 fold change [up and down]). The pattern of gene expression suggests that lung recovery is characterized by enhanced cell division, vascularization, wound healing and adjustment of host adaptive immune responses. Proliferation assay by PCNA staining further confirmed that at day 3 lungs have significantly higher cell proliferation than at day 1. Furthermore, at day 3 lungs displayed significantly lower levels of vascular permeability to albumin, compared to day 1. Collectively, this data helps us elucidate the molecular mechanisms of the recovery after MRSA lung infection.

Original languageEnglish (US)
Article numbere70176
JournalPLoS One
Volume8
Issue number8
DOIs
StatePublished - Aug 1 2013
Externally publishedYes

Fingerprint

Methicillin-Resistant Staphylococcus aureus
pneumonia
Pneumonia
lungs
Recovery
Lung
mice
Cell proliferation
Infection
Gene expression
infection
Fluids
Histology
Methicillin
Proliferating Cell Nuclear Antigen
Microarrays
Bronchoalveolar Lavage Fluid
Albumins
Assays
Complementary DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Chen, J., Feng, G., Guo, Q., Wardenburg, J. B., Lin, S., Inoshima, I., ... Wunderink, R. G. (2013). Transcriptional Events during the Recovery from MRSA Lung Infection: A Mouse Pneumonia Model. PLoS One, 8(8), [e70176]. https://doi.org/10.1371/journal.pone.0070176

Transcriptional Events during the Recovery from MRSA Lung Infection : A Mouse Pneumonia Model. / Chen, Jiwang; Feng, Gang; Guo, Qiang; Wardenburg, Juliane B.; Lin, Simon; Inoshima, Ichiro; Deaton, Ryan; Yuan, Jason X.J.; Garcia, Joe G.N.; Machado, Roberto; Otto, Michael; Wunderink, Richard G.

In: PLoS One, Vol. 8, No. 8, e70176, 01.08.2013.

Research output: Contribution to journalArticle

Chen, J, Feng, G, Guo, Q, Wardenburg, JB, Lin, S, Inoshima, I, Deaton, R, Yuan, JXJ, Garcia, JGN, Machado, R, Otto, M & Wunderink, RG 2013, 'Transcriptional Events during the Recovery from MRSA Lung Infection: A Mouse Pneumonia Model', PLoS One, vol. 8, no. 8, e70176. https://doi.org/10.1371/journal.pone.0070176
Chen, Jiwang ; Feng, Gang ; Guo, Qiang ; Wardenburg, Juliane B. ; Lin, Simon ; Inoshima, Ichiro ; Deaton, Ryan ; Yuan, Jason X.J. ; Garcia, Joe G.N. ; Machado, Roberto ; Otto, Michael ; Wunderink, Richard G. / Transcriptional Events during the Recovery from MRSA Lung Infection : A Mouse Pneumonia Model. In: PLoS One. 2013 ; Vol. 8, No. 8.
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