Transcriptional profile of right ventricular tissue during acute pulmonary embolism in rats

John Zagorski, Nina Sanapareddy, Michael A. Gellar, Jeffrey Kline, John A. Watts

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Acute pulmonary embolism (PE) is the third leading cause of cardiovascular death in the United States. Moderate to severe PE can cause pulmonary arterial hypertension (PH) with resultant right ventricular (RV) heart damage. The mechanisms leading to RV failure after PE are not well defined, although it is becoming clear that PH-induced inflammatory responses are involved. We previously demonstrated profound neutrophil-mediated inflammation and RV dysfunction during PE that was associated with increased expression of several chemokine genes. However, a complete assessment of transcriptional changes in RVs during PE is still lacking. We have now used DNA microarrays to assess the alterations in gene expression in RV tissue during acute PE/PH in rats. Key results were confirmed with real-time RT-PCR. Nine CC-chemokine genes (CCL-2, -3, -4, -6, -7, -9, -17, -20, -27), five CXC-chemokine genes (CXCL-1, -2, -9, -10, -16), and the receptors CCR1 and CXCR4 were upregulated after 18 h of moderate PE, while one C-chemokine (XCL-1) and one CXC-chemokine (CXCL-12) were downregulated. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated increased expression of many inflammatory genes. There was also a major shift in the expression of components of metabolic pathways, including downregulation of fatty acid transporters and oxidative enzymes, a change in glucose transporters, and upregulation of stretch-sensing and hypoxia-inducible transcription factors. This pattern suggests an extensive shift in cardiac physiology favoring the expression of the "fetal gene program.".

Original languageEnglish (US)
Pages (from-to)101-111
Number of pages11
JournalPhysiological Genomics
Volume34
Issue number1
DOIs
StatePublished - Jun 2008
Externally publishedYes

Fingerprint

Pulmonary Embolism
Pulmonary Hypertension
CXC Chemokines
Genes
CCR1 Receptors
Down-Regulation
C Chemokines
Encyclopedias
CXCR4 Receptors
Right Ventricular Dysfunction
Gene Expression
CC Chemokines
Gene Ontology
Facilitative Glucose Transport Proteins
Metabolic Networks and Pathways
Oligonucleotide Array Sequence Analysis
Chemokines
Real-Time Polymerase Chain Reaction
Cause of Death
Neutrophils

Keywords

  • GeneSifter
  • Heart
  • Inflammation
  • Microarray
  • Pulmonary hypertension

ASJC Scopus subject areas

  • Physiology
  • Genetics

Cite this

Transcriptional profile of right ventricular tissue during acute pulmonary embolism in rats. / Zagorski, John; Sanapareddy, Nina; Gellar, Michael A.; Kline, Jeffrey; Watts, John A.

In: Physiological Genomics, Vol. 34, No. 1, 06.2008, p. 101-111.

Research output: Contribution to journalArticle

Zagorski, John ; Sanapareddy, Nina ; Gellar, Michael A. ; Kline, Jeffrey ; Watts, John A. / Transcriptional profile of right ventricular tissue during acute pulmonary embolism in rats. In: Physiological Genomics. 2008 ; Vol. 34, No. 1. pp. 101-111.
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