Transcriptional regulation of the mouse CSF-1 gene

Maureen Harrington, Bruce W. Konicek, Xiao Ling Xia, An Song

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Research in our laboratory is aimed at understanding the cellular and molecular mechanisms that govern colony stimulating factor-1 (CSF-1) gene expression. Our hypothesis is that a basal set of trans-acting factors is bound to the CSF-1 gene during fibroblast proliferation, resulting in constitutive CSF-1 gene expression. Modulation of CSF-1 gene transcription by growth-arrest (decrease) or stimulation of growth-arrested fibroblasts (re- initiate) is mediated by changes in the basal set of factors bound and/or by the addition of stimulus-specific factors. We have extended our hypothesis to include other cell types (monocytes) to determine if mechanisms used to control CSF-1 gene expression in fibroblasts are unique or represent common nontissue-specific regulatory mechanisms. Analysis of CSF-1 CAT reporter constructs in transiently transfected fibroblasts and monocytes was used to identify CSF-1 genomic sequences that affect transcriptional activity. DNase I protection, electrophoretic mobility shift, and methylation interference assays were used to identify the putative cis-acting elements. Results of our study suggest multiple trans-acting factors may regulate CSF-1 gene expression; some may be tissue specific, while others, such as AP1, CTF/NF1, Sp1, and Sp3, are shared in common.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalMolecular Reproduction and Development
Volume46
Issue number1
DOIs
StatePublished - Jan 1997

Fingerprint

Macrophage Colony-Stimulating Factor
Genes
Fibroblasts
Gene Expression
Trans-Activators
Monocytes
Deoxyribonuclease I
Growth
Methylation
Research

Keywords

  • cytokines
  • transcription factors

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Transcriptional regulation of the mouse CSF-1 gene. / Harrington, Maureen; Konicek, Bruce W.; Xia, Xiao Ling; Song, An.

In: Molecular Reproduction and Development, Vol. 46, No. 1, 01.1997, p. 39-45.

Research output: Contribution to journalArticle

Harrington, Maureen ; Konicek, Bruce W. ; Xia, Xiao Ling ; Song, An. / Transcriptional regulation of the mouse CSF-1 gene. In: Molecular Reproduction and Development. 1997 ; Vol. 46, No. 1. pp. 39-45.
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