Transcriptional regulation of the mouse CSF-1 gene

Maureen Harrington, Bruce W. Konicek, Xiao Ling Xia, An Song

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Research in our laboratory is aimed at understanding the cellular and molecular mechanisms that govern colony stimulating factor-1 (CSF-1) gene expression. Our hypothesis is that a basal set of trans-acting factors is bound to the CSF-1 gene during fibroblast proliferation, resulting in constitutive CSF-1 gene expression. Modulation of CSF-1 gene transcription by growth-arrest (decrease) or stimulation of growth-arrested fibroblasts (re- initiate) is mediated by changes in the basal set of factors bound and/or by the addition of stimulus-specific factors. We have extended our hypothesis to include other cell types (monocytes) to determine if mechanisms used to control CSF-1 gene expression in fibroblasts are unique or represent common nontissue-specific regulatory mechanisms. Analysis of CSF-1 CAT reporter constructs in transiently transfected fibroblasts and monocytes was used to identify CSF-1 genomic sequences that affect transcriptional activity. DNase I protection, electrophoretic mobility shift, and methylation interference assays were used to identify the putative cis-acting elements. Results of our study suggest multiple trans-acting factors may regulate CSF-1 gene expression; some may be tissue specific, while others, such as AP1, CTF/NF1, Sp1, and Sp3, are shared in common.

Original languageEnglish (US)
Pages (from-to)39-45
Number of pages7
JournalMolecular Reproduction and Development
Volume46
Issue number1
DOIs
StatePublished - Jan 1 1997

Keywords

  • cytokines
  • transcription factors

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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