Transcriptional terminators of RNA polymerase II are associated with yeast replication origins

Shaoxiong Chen, Roxanne Reger, Charles Miller, Linda E. Hyman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The compact organization of the Saccharomyces cerevisiae genome necessitates that non-coding regulatory sequences reside in close proximity to one another. Here we show there is an intimate association between transcription terminators and DNA replication origins. Four replication origins were analyzed in a reporter gene assay that detects sequences that direct 3' end formation of mRNA transcripts. All four replication origins function as orientation-independent transcription terminators in this system, producing truncated polyadenylated mRNAs. Despite this close association, the cis-acting elements that confer replication origin function are genetically separable from those required for transcription termination. Several models are explored in an attempt to address how and why the signals specifying transcription termination and replication initiation overlap.

Original languageEnglish (US)
Pages (from-to)2885-2893
Number of pages9
JournalNucleic Acids Research
Volume24
Issue number15
DOIs
StatePublished - 1996
Externally publishedYes

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Replication Origin
RNA Polymerase II
Yeasts
Messenger RNA
DNA Replication
Reporter Genes
Saccharomyces cerevisiae
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Transcriptional terminators of RNA polymerase II are associated with yeast replication origins. / Chen, Shaoxiong; Reger, Roxanne; Miller, Charles; Hyman, Linda E.

In: Nucleic Acids Research, Vol. 24, No. 15, 1996, p. 2885-2893.

Research output: Contribution to journalArticle

Chen, Shaoxiong ; Reger, Roxanne ; Miller, Charles ; Hyman, Linda E. / Transcriptional terminators of RNA polymerase II are associated with yeast replication origins. In: Nucleic Acids Research. 1996 ; Vol. 24, No. 15. pp. 2885-2893.
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