Transduction of human IL-9 receptor cDNA into TF1 cells induces IL-9 dependency and erythroid differentiation

Mang Xiao, Zaiming Luo, Charlie Mantel, Hal Broxmeyer, Li Lu

Research output: Contribution to journalArticle

Abstract

Human growth factor-dependent cell line TF1, which lacks interleukin (IL)-9 receptors (R) and does not grow in IL-9, was transduced with a retroviral vector containing human IL-9R cDNA and a selection marker. An IL- 9-dependent TF1 cell line, which could also grow in other cytokines, was established after selection in G418 and could produce mature RBC in response to cytokine stimulation. TF1 cells transduced with the same viral vector without the IL-9R insert cDNA (mock control) and then selected responded the same as nontransduced TF1 cells. They failed to grow in response to IL-9 and did not generate RBC. An increased number and size of burst-forming units- erythroid (BFU-E)-like colonies were detected from IL-9R-transduced TF1 cells, compared with mock-transduced cells, in response to erythropoietin (EPO) and IL-9. To evaluate self-renewal and differentiation capacity, colony-replating assays were performed in the presence of IL-3, GM-CSF, IL-9, and EPO. After four replatings, the cloning efficiency of IL-9R-transduced TF1 cells decreased from 98% to 38%, most likely due to terminal erythroid cell differentiation. In contrast, no change in replating efficiency was detected in mock-transduced cells. TF1 cells stably expressing IL-9R and responding to IL-9 can serve as a cell line model to study the intracellular signals mediating IL-9-induced erythroid cell proliferation and differentiation.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalJournal of Hematotherapy and Stem Cell Research
Volume9
Issue number1
DOIs
StatePublished - 2000

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Interleukin-9
Interleukins
Complementary DNA
Erythroid Cells
Erythropoietin
Cell Line
Interleukin-9 Receptors
Cell Differentiation
Cytokines
Erythroid Precursor Cells
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
human IL9R protein
Organism Cloning
Intercellular Signaling Peptides and Proteins
Cell Proliferation

ASJC Scopus subject areas

  • Hematology
  • Immunology

Cite this

Transduction of human IL-9 receptor cDNA into TF1 cells induces IL-9 dependency and erythroid differentiation. / Xiao, Mang; Luo, Zaiming; Mantel, Charlie; Broxmeyer, Hal; Lu, Li.

In: Journal of Hematotherapy and Stem Cell Research, Vol. 9, No. 1, 2000, p. 77-82.

Research output: Contribution to journalArticle

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