Transduction of human interleukin-9 receptor gene into human cord blood erythroid progenitors increases the number of erythropoietin-dependent erythroid colonies

M. Xiao, Y. C. Yang, L. Yang, M. S. Chang, K. Cornetta, H. E. Broxmeyer, L. Lu

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

We constructed a retroviral vector, pLhIL-9RSN, containing cDNA encoding the human interleukin-9 receptor (IL-9R) along with a neomycin phosphotransferase gene (Neo). In order to study the biological effects of the IL-9R, high titer (1-5 x 105 CFU/ml) viral supernatant, generated from the packaging cell lines, ecotropic GPE86 and amphotropic PA317, was used to transduce the IL-9R gene into sorted populations of CD34++CD33- cells from human cord blood which are highly enriched for erythroid progenitor cells (BFU-E). Colony formation by BFU-E transduced with the IL-9R gene and grown without selection in G418 and in the presence of erythropoietin (Epo) and interleukin (IL)-9 was significantly increased up to three-fold and the size of the erythroid colonies was significantly increased 50-100% compared to colony formation by mock virus transduced cells. Moreover, colony formation by IL-9R-transduced cells was more sensitive to stimulation with lower doses of IL-9 and Epo. Individual colonies formed with or without selection in G418 were evaluated. Proviral integration and mRNA expression were respectively assessed by polymerase chain reaction (PCR) and reverse transcriptase (RT) PCR analysis and were apparent in 93% and 84% of the G418-resistant colonies and 52% and 48% of the colonies grown in the absence of G418. Our study demonstrates that a functional human IL-9R gene can be efficiently transduced into human cord blood hematopoietic progenitors using retroviral vectors with increased cytokine-dependent erythroid colony formation.

Original languageEnglish (US)
Pages (from-to)1103-1109
Number of pages7
JournalBone marrow transplantation
Volume18
Issue number6
StatePublished - Dec 1 1996

Keywords

  • Erythroid progenitors
  • IL-9 receptor gene
  • Transduction

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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