Transduktion Primärer Humaner T-Zellen mit einem CD44v7/ 8-Spezifischen T-Zellrezeptor

Translated title of the contribution: Transduction of Human T-Cells Targeting CD44v7/8-Presenting Cells

M. A. Stoff-Khalili, I. Herrmann, C. Nestle-Krämling, H. Hallscheidt, D. Niederacher, H. Hanenberg, H. G. Bender, P. Dall

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: The variant epitope CD44v7/8 is frequently expressed on cervical carcinoma. Therefore CD44vT/8 is a tumour-specific antigen and may play a role as a promising target for tumour-specific immunotherapy. Since many gene transfer methods in human T-cells are limited and problematical, the primary aim of our study was to develop a method for the transduction of T-lymphocytes with a CD44vT/8 target specifity. Material and Methods: The genes coding for the single chain fragment scFv of the mononuclear antibody VFF 17 and of the ζ-chain of the TCR (T-cell receptor) complex were fused and inserted into a retroviral vector. We retrovirally transduced primary human T-cells. The gene transfer was tested by PCR (polymerase chain reaction). A FACS (fluorescent activated cell sorter) and a functionality test were performed to investigate the expression of the chimeric receptor of primary human T-cells. Results: The mRNA expression of the chimeric receptor on the transduced human T-cells could be demonstrated. But on the protein level no expression of the scFv(VFF 17)y:α:ζ-fusion protein could be detected. Discussion: Further studies will show whether the undetected expression of the surface protein on the transduced T-cells is due to a pseudotransduction, a not performed translation or an incorrect folding of the protein and consequently a flawed transportation of the protein to the surface.

Translated title of the contributionTransduction of Human T-Cells Targeting CD44v7/8-Presenting Cells
Original languageGerman
Pages (from-to)279-285
Number of pages7
JournalGeburtshilfe und Frauenheilkunde
Volume64
Issue number3
DOIs
StatePublished - Mar 1 2004

Keywords

  • CD44v7/8
  • Cervical carcinoma
  • Gene therapy
  • T-cells

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Maternity and Midwifery

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