Transduction of recombinant human erythropoietin receptor cDNA into daughter progenitors derived from single CD343+ cord blood cells changes the differentiation profile of daughter progenitors

Li Lu, Zhi Hua Li, Jie He, Yue Ge, Susan Rice, Hal Broxmeyer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this study, we tested the capacity to change the differentiation profile of progenitor cells by retroviral-mediated transduction of EpoR cDNA into one of the paired daughter cells derived from single CD343+ CB cells. Our results show that for the non-viral-treated daughter cells, the majority (99.6%) formed the same colony type. However, with cells transduced with viral vectors, 7.1% of the daughter cells transduced with the EpoR cDNA formed either a burst forming unit-erythroid (BFU-E) or a colony-forming unit-granulocyte, macrophage, erythroid, megakaryocyte (CFU-GEMM) colony compared to the other daughter cell transduced with viral supernatant lacking EpoR cDNA, which formed either a colony-forming unit granulocyte-macrophage (CFU-GM) or a high proliferative potential-colony forming cell (HPP-CFC) colony. Expression of the transduced EpoR cDNA was confirmed in individual colonies by RT-PCR analysis. These results substantiate in a more rigorous fashion our previous results that it is possible to change the Epo-responsive differentiation profile of progenitor cells by transduction into these cells of an EpoR cDNA and this change was apparent only in daughter cells derived from single CD343+ kit+ cells transduced with EpoR cDNA.

Original languageEnglish
Pages (from-to)389-394
Number of pages6
JournalJournal of Leukocyte Biology
Volume63
Issue number3
StatePublished - Mar 1998

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Erythropoietin Receptors
Fetal Blood
Cell Differentiation
Blood Cells
Complementary DNA
Stem Cells
Myeloid Progenitor Cells
Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells

Keywords

  • Erythroid differentiation
  • Viral vectors

ASJC Scopus subject areas

  • Cell Biology

Cite this

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title = "Transduction of recombinant human erythropoietin receptor cDNA into daughter progenitors derived from single CD343+ cord blood cells changes the differentiation profile of daughter progenitors",
abstract = "In this study, we tested the capacity to change the differentiation profile of progenitor cells by retroviral-mediated transduction of EpoR cDNA into one of the paired daughter cells derived from single CD343+ CB cells. Our results show that for the non-viral-treated daughter cells, the majority (99.6{\%}) formed the same colony type. However, with cells transduced with viral vectors, 7.1{\%} of the daughter cells transduced with the EpoR cDNA formed either a burst forming unit-erythroid (BFU-E) or a colony-forming unit-granulocyte, macrophage, erythroid, megakaryocyte (CFU-GEMM) colony compared to the other daughter cell transduced with viral supernatant lacking EpoR cDNA, which formed either a colony-forming unit granulocyte-macrophage (CFU-GM) or a high proliferative potential-colony forming cell (HPP-CFC) colony. Expression of the transduced EpoR cDNA was confirmed in individual colonies by RT-PCR analysis. These results substantiate in a more rigorous fashion our previous results that it is possible to change the Epo-responsive differentiation profile of progenitor cells by transduction into these cells of an EpoR cDNA and this change was apparent only in daughter cells derived from single CD343+ kit+ cells transduced with EpoR cDNA.",
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AU - Li, Zhi Hua

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AU - Ge, Yue

AU - Rice, Susan

AU - Broxmeyer, Hal

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