Transduction of wild-type merlin into human schwannoma cells decreases schwannoma cell growth and induces apoptosis

K. M M Schulze, C. O. Hanemann, H. W. Müller, H. Hanenberg

Research output: Contribution to journalArticle

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Abstract

Mutations in both alleles of the tumour suppressor gene coding for merlin/schwannomin, an ERM family protein, cause the hereditary disease neurofibromatosis type 2 (NF2). NF2 is characterized by the development of multiple nervous system tumours especially vestibular schwannomas. Efficient oncoretrovirus-mediated gene transfer of different merlin constructs was used to stably re-express wild-type merlin in primary cells derived from human schwannomas. Using two-parameters FACS analysis we show that expression of wild-type merlin in NF2 cells led to significant reduction of proliferation and G0/G1 arrest in transduced schwannoma cells. In addition, we show increased apoptosis of schwannoma cells transduced with wild-type merlin. Our findings in primary schwannoma cells from NF2 patients strongly support the hypothesis of merlin acting as a tumour suppressor and may help in understanding development of human schwannomas in NF2.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalHuman Molecular Genetics
Volume11
Issue number1
StatePublished - Jan 1 2002
Externally publishedYes

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Neurofibromin 2
Neurilemmoma
Neurofibromatosis 2
Apoptosis
Growth
Nervous System Neoplasms
Inborn Genetic Diseases
Acoustic Neuroma
Human Development
Tumor Suppressor Genes
Alleles
Mutation

ASJC Scopus subject areas

  • Genetics

Cite this

Schulze, K. M. M., Hanemann, C. O., Müller, H. W., & Hanenberg, H. (2002). Transduction of wild-type merlin into human schwannoma cells decreases schwannoma cell growth and induces apoptosis. Human Molecular Genetics, 11(1), 69-76.

Transduction of wild-type merlin into human schwannoma cells decreases schwannoma cell growth and induces apoptosis. / Schulze, K. M M; Hanemann, C. O.; Müller, H. W.; Hanenberg, H.

In: Human Molecular Genetics, Vol. 11, No. 1, 01.01.2002, p. 69-76.

Research output: Contribution to journalArticle

Schulze, KMM, Hanemann, CO, Müller, HW & Hanenberg, H 2002, 'Transduction of wild-type merlin into human schwannoma cells decreases schwannoma cell growth and induces apoptosis', Human Molecular Genetics, vol. 11, no. 1, pp. 69-76.
Schulze, K. M M ; Hanemann, C. O. ; Müller, H. W. ; Hanenberg, H. / Transduction of wild-type merlin into human schwannoma cells decreases schwannoma cell growth and induces apoptosis. In: Human Molecular Genetics. 2002 ; Vol. 11, No. 1. pp. 69-76.
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