Transfer of a lincomycin-resistant plasmid between coagulase-negative staphylococci during soybean fermentation and mouse intestine passage

Sojeong Heo, Taeok Bae, Jong Hoon Lee, Do Won Jeong

Research output: Contribution to journalArticle

Abstract

Staphylococcus equorum is a benign bacterium and the predominant species in high-salt fermented food. Some strains of S. equorum contain antibiotic-resistance plasmids, such as pSELNU1 that contains a lincosamide nucleotidyltransferase (lnuA) gene and confers resistance to lincomycin. Previously, we showed that pSELNU1 is transferred to other bacteria under laboratory growth conditions. However, it is not known if the plasmid can be transferred to other bacteria during food fermentation (in situ) or during passage through animal intestines (in vivo). In this study, we examined the in situ and in vivo transfer of pSELNU1 using Staphylococcus saprophyticus as a recipient. During soybean fermentation, pSELNU1 was transferred to S. saprophyticus at a rate of 1.9 × 10-5-5.6 × 10-6 per recipient in the presence of lincomycin. However, during passage through murine intestines, the plasmid was transferred at similar rates (1.3 × 10-5 per recipient) in the absence of lincomycin, indicating that the plasmid transfer is much more efficient under in vivo conditions. Based on these results, we conclude that it is prudent to examine food fermentation starter candidates for the presence of mobile genetic elements containing antibiotic resistance genes and to select candidates lacking these genes.

Original languageEnglish (US)
Article numberfnz113
JournalFEMS Microbiology Letters
Volume366
Issue number10
DOIs
StatePublished - Jun 10 2019

Fingerprint

Lincomycin
Coagulase
Staphylococcus
Soybeans
Fermentation
Intestines
Plasmids
Staphylococcus saprophyticus
Microbial Drug Resistance
Bacteria
Food
Nucleotidyltransferases
Lincosamides
Interspersed Repetitive Sequences
Genes
Salts
Growth

Keywords

  • Horizontal gene transfer
  • lincomycin
  • pSELNU1
  • Staphylococcus equorum
  • Staphylococcus saprophyticus
  • starter candidate

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Transfer of a lincomycin-resistant plasmid between coagulase-negative staphylococci during soybean fermentation and mouse intestine passage. / Heo, Sojeong; Bae, Taeok; Lee, Jong Hoon; Jeong, Do Won.

In: FEMS Microbiology Letters, Vol. 366, No. 10, fnz113, 10.06.2019.

Research output: Contribution to journalArticle

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