Transferrin and the trophic effect of neural tissue on amphibian limb regeneration blastemas

Syeda Iffat Munaim, Anthony Mescher

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Nerves promote regeneration of amputated urodele limbs, but the chemical basis of the effect is not known. We have examined the possible involvement of the iron-transport factor transferrin, which is important for cell proliferation and is present in vertebrate nervous tissue. Newt brain extract stimulated incorporation of [3H]thymidine in cultured blastemas from regenerating newt forelimbs, showing a biphasic dose-response similar to that of heterologous transferrin. As shown previously for transferrin, the inhibitory effect of brain extract at high concentrations was relieved by the addition of iron. Activity of brain extract was reduced by treatment with an iron-chelating agent and fully restored by the readdition of iron. Double immunodiffusion of newt tissue extracts and antibodies against newt plasma transferrin demonstrated the presence of transferrin-like factors in brain, spinal cord, and peripheral nerve. These results indicate that activity of transferrin may be part of the trophic effect of brain extract on cultured blastemas.

Original languageEnglish
Pages (from-to)138-142
Number of pages5
JournalDevelopmental Biology
Volume116
Issue number1
DOIs
StatePublished - 1986

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Amphibians
Transferrin
Salamandridae
Regeneration
Extremities
Brain
Iron
Iron Chelating Agents
Nerve Tissue
Spinal Nerves
Nerve Regeneration
Tissue Extracts
Forelimb
Immunodiffusion
Peripheral Nerves
Thymidine
Vertebrates
Spinal Cord
Cell Proliferation
Antibodies

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Transferrin and the trophic effect of neural tissue on amphibian limb regeneration blastemas. / Munaim, Syeda Iffat; Mescher, Anthony.

In: Developmental Biology, Vol. 116, No. 1, 1986, p. 138-142.

Research output: Contribution to journalArticle

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