Transgenic expression of nonclassically secreted FGF suppresses kidney repair

Aleksandr Kirov, Maria Duarte, Justin Guay, Michele Karolak, Cong Yan, Leif Oxburgh, Igor Prudovsky

Research output: Contribution to journalArticle

8 Scopus citations


FGF1 is a signal peptide-less nonclassically released growth factor that is involved in angiogenesis, tissue repair, inflammation, and carcinogenesis. The effects of nonclassical FGF export in vivo are not sufficiently studied. We produced transgenic mice expressing FGF1 in endothelial cells (EC), which allowed the detection of FGF1 export to the vasculature, and studied the efficiency of postischemic kidney repair in these animals. Although FGF1 transgenic mice had a normal phenotype with unperturbed kidney structure, they showed a severely inhibited kidney repair after unilateral ischemia/reperfusion. This was manifested by a strong decrease of postischemic kidney size and weight, whereas the undamaged contralateral kidney exhibited an enhanced compensatory size increase. In addition, the postischemic kidneys of transgenic mice were characterized by hyperplasia of interstitial cells, paucity of epithelial tubular structures, increase of the areas occupied by connective tissue, and neutrophil and macrophage infiltration. The continuous treatment of transgenic mice with the cell membrane stabilizer, taurine, inhibited nonclassical FGF1 export and significantly rescued postischemic kidney repair. It was also found that similar to EC, the transgenic expression of FGF1 in monocytes and macrophages suppresses kidney repair. We suggest that nonclassical export may be used as a target for the treatment of pathologies involving signal peptide-less FGFs.

Original languageEnglish (US)
Article numbere36485
JournalPloS one
Issue number5
StatePublished - May 14 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Kirov, A., Duarte, M., Guay, J., Karolak, M., Yan, C., Oxburgh, L., & Prudovsky, I. (2012). Transgenic expression of nonclassically secreted FGF suppresses kidney repair. PloS one, 7(5), [e36485].