Transition Zone in Total Colonic Aganglionosis and Colorectal Hirschsprung’s Disease Shows a Similar Trend of Mucosal Innervation: Image Processing and Analysis Study

Fernanda Cordeiro-Rudnisky, Sangtae Ahn, Natallia Sheuka, Christine Whyte, Ann Boguniewicz, Rong Fan, Jingmei Lin, Timothy A. Jennings, Hwajeong Lee

Research output: Contribution to journalArticle

Abstract

The aganglionic segment of bowel in Hirschsprung’s disease (HD) varies in length. It is not clear whether total colonic aganglionosis (TCA) merely represents a long form of HD or a different phenotype of the disease. Animal model studies suggest that TCA may have a longer transition zone (TZ) than conventional colorectal HD. We compared mucosal innervation of TZ in 2 TCA cases and 10 conventional colorectal HD cases by quantifying calretinin-positive mucosal nerve fibers using image processing and analysis. One TCA was associated with esophageal atresia-tracheoesophageal fistula, the other with trisomy 21. The gradients of calretinin-stained pixel count increase per distance from the beginning of TZ (slope) for TCA were not significantly different from those for the conventional HD group. Given this observation, it is speculated that the length of TZ in TCA may fall within the range of and may not be much longer than conventional colorectal HD.

Original languageEnglish (US)
JournalPediatric and Developmental Pathology
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

Hirschsprung Disease
Calbindin 2
Down Syndrome
Nerve Fibers
Animal Models

Keywords

  • calretinin
  • Hirschsprung
  • image processing and analysis
  • total colonic aganglionosis
  • tracheoesophageal fistula
  • transition zone

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Pathology and Forensic Medicine

Cite this

Transition Zone in Total Colonic Aganglionosis and Colorectal Hirschsprung’s Disease Shows a Similar Trend of Mucosal Innervation : Image Processing and Analysis Study. / Cordeiro-Rudnisky, Fernanda; Ahn, Sangtae; Sheuka, Natallia; Whyte, Christine; Boguniewicz, Ann; Fan, Rong; Lin, Jingmei; Jennings, Timothy A.; Lee, Hwajeong.

In: Pediatric and Developmental Pathology, 01.01.2019.

Research output: Contribution to journalArticle

@article{58e2219b16d743a9844066950ac48c92,
title = "Transition Zone in Total Colonic Aganglionosis and Colorectal Hirschsprung’s Disease Shows a Similar Trend of Mucosal Innervation: Image Processing and Analysis Study",
abstract = "The aganglionic segment of bowel in Hirschsprung’s disease (HD) varies in length. It is not clear whether total colonic aganglionosis (TCA) merely represents a long form of HD or a different phenotype of the disease. Animal model studies suggest that TCA may have a longer transition zone (TZ) than conventional colorectal HD. We compared mucosal innervation of TZ in 2 TCA cases and 10 conventional colorectal HD cases by quantifying calretinin-positive mucosal nerve fibers using image processing and analysis. One TCA was associated with esophageal atresia-tracheoesophageal fistula, the other with trisomy 21. The gradients of calretinin-stained pixel count increase per distance from the beginning of TZ (slope) for TCA were not significantly different from those for the conventional HD group. Given this observation, it is speculated that the length of TZ in TCA may fall within the range of and may not be much longer than conventional colorectal HD.",
keywords = "calretinin, Hirschsprung, image processing and analysis, total colonic aganglionosis, tracheoesophageal fistula, transition zone",
author = "Fernanda Cordeiro-Rudnisky and Sangtae Ahn and Natallia Sheuka and Christine Whyte and Ann Boguniewicz and Rong Fan and Jingmei Lin and Jennings, {Timothy A.} and Hwajeong Lee",
year = "2019",
month = "1",
day = "1",
doi = "10.1177/1093526619864225",
language = "English (US)",
journal = "Pediatric and Developmental Pathology",
issn = "1093-5266",
publisher = "Society for Pediatric Pathology",

}

TY - JOUR

T1 - Transition Zone in Total Colonic Aganglionosis and Colorectal Hirschsprung’s Disease Shows a Similar Trend of Mucosal Innervation

T2 - Image Processing and Analysis Study

AU - Cordeiro-Rudnisky, Fernanda

AU - Ahn, Sangtae

AU - Sheuka, Natallia

AU - Whyte, Christine

AU - Boguniewicz, Ann

AU - Fan, Rong

AU - Lin, Jingmei

AU - Jennings, Timothy A.

AU - Lee, Hwajeong

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The aganglionic segment of bowel in Hirschsprung’s disease (HD) varies in length. It is not clear whether total colonic aganglionosis (TCA) merely represents a long form of HD or a different phenotype of the disease. Animal model studies suggest that TCA may have a longer transition zone (TZ) than conventional colorectal HD. We compared mucosal innervation of TZ in 2 TCA cases and 10 conventional colorectal HD cases by quantifying calretinin-positive mucosal nerve fibers using image processing and analysis. One TCA was associated with esophageal atresia-tracheoesophageal fistula, the other with trisomy 21. The gradients of calretinin-stained pixel count increase per distance from the beginning of TZ (slope) for TCA were not significantly different from those for the conventional HD group. Given this observation, it is speculated that the length of TZ in TCA may fall within the range of and may not be much longer than conventional colorectal HD.

AB - The aganglionic segment of bowel in Hirschsprung’s disease (HD) varies in length. It is not clear whether total colonic aganglionosis (TCA) merely represents a long form of HD or a different phenotype of the disease. Animal model studies suggest that TCA may have a longer transition zone (TZ) than conventional colorectal HD. We compared mucosal innervation of TZ in 2 TCA cases and 10 conventional colorectal HD cases by quantifying calretinin-positive mucosal nerve fibers using image processing and analysis. One TCA was associated with esophageal atresia-tracheoesophageal fistula, the other with trisomy 21. The gradients of calretinin-stained pixel count increase per distance from the beginning of TZ (slope) for TCA were not significantly different from those for the conventional HD group. Given this observation, it is speculated that the length of TZ in TCA may fall within the range of and may not be much longer than conventional colorectal HD.

KW - calretinin

KW - Hirschsprung

KW - image processing and analysis

KW - total colonic aganglionosis

KW - tracheoesophageal fistula

KW - transition zone

UR - http://www.scopus.com/inward/record.url?scp=85071020095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071020095&partnerID=8YFLogxK

U2 - 10.1177/1093526619864225

DO - 10.1177/1093526619864225

M3 - Article

AN - SCOPUS:85071020095

JO - Pediatric and Developmental Pathology

JF - Pediatric and Developmental Pathology

SN - 1093-5266

ER -