Transmigration of human CD34+ cells

Seiji Fukuda, Louis Pelus

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Understanding mechanisms responsible for engraftment of hematopoietic stem cells (HSC) is important to achieve successful HSC transplantation. Homing of HSC to the bone marrow niche is believed to be a crucial step for engraftment. However, the molecular mechanisms that regulate HSC homing are not understood well. Migration of HSC in response to cytokines and chemokines is believed to be one of the critical steps of HSC homing and mobilization. Evaluating the migration of HSC will help to understand the mechanisms responsible for their homing and/or mobilization. In this chapter, we will describe the methodology utilized in our laboratory to evaluate migration of human CD34+ cells that contain HSC.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages55-75
Number of pages21
Volume430
StatePublished - Aug 31 2007
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume430
ISSN (Print)10643745

Fingerprint

Hematopoietic Stem Cells
Hematopoietic Stem Cell Mobilization
Hematopoietic Stem Cell Transplantation
Chemokines
Bone Marrow
Cytokines

Keywords

  • CD34 cells
  • Chemotaxis assay
  • SDF1/CXCR4
  • Transmigration

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Fukuda, S., & Pelus, L. (2007). Transmigration of human CD34+ cells. In Methods in Molecular Biology (Vol. 430, pp. 55-75). (Methods in Molecular Biology; Vol. 430).

Transmigration of human CD34+ cells. / Fukuda, Seiji; Pelus, Louis.

Methods in Molecular Biology. Vol. 430 2007. p. 55-75 (Methods in Molecular Biology; Vol. 430).

Research output: Chapter in Book/Report/Conference proceedingChapter

Fukuda, S & Pelus, L 2007, Transmigration of human CD34+ cells. in Methods in Molecular Biology. vol. 430, Methods in Molecular Biology, vol. 430, pp. 55-75.
Fukuda S, Pelus L. Transmigration of human CD34+ cells. In Methods in Molecular Biology. Vol. 430. 2007. p. 55-75. (Methods in Molecular Biology).
Fukuda, Seiji ; Pelus, Louis. / Transmigration of human CD34+ cells. Methods in Molecular Biology. Vol. 430 2007. pp. 55-75 (Methods in Molecular Biology).
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