Transmigration of human CD34+ cells

Seiji Fukuda, Louis M. Pelus

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Understanding mechanisms responsible for engraftment of hematopoietic stem cells (HSC) is important to achieve successful HSC transplantation. Homing of HSC to the bone marrow niche is believed to be a crucial step for engraftment. However, the molecular mechanisms that regulate HSC homing are not understood well. Migration of HSC in response to cytokines and chemokines is believed to be one of the critical steps of HSC homing and mobilization. Evaluating the migration of HSC will help to understand the mechanisms responsible for their homing and/or mobilization. In this chapter, we will describe the methodology utilized in our laboratory to evaluate migration of human CD34+ cells that contain HSC.

Original languageEnglish (US)
Title of host publicationHematopoietic Stem Cell Protocols
Pages55-75
Number of pages21
StatePublished - Aug 31 2007

Publication series

NameMethods in Molecular Biology
Volume430
ISSN (Print)1064-3745

    Fingerprint

Keywords

  • CD34 cells
  • Chemotaxis assay
  • SDF1/CXCR4
  • Transmigration

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Fukuda, S., & Pelus, L. M. (2007). Transmigration of human CD34+ cells. In Hematopoietic Stem Cell Protocols (pp. 55-75). (Methods in Molecular Biology; Vol. 430).