Transplantation of cerebellar anlagen to hosts with genetic cerebellocortical atrophy

Lazaros C. Triarhou, Walter C. Low, Bernardino Ghetti

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Embryonic cerebellar grafts from genetically normal donors were implanted into the cerebellomedullary cistern of adult 'Purkinje cell degeneration' (pcd) and weaver mutant mice, which are respectively characterized by the selective loss of Purkinje and granule cells. Grafts placed into both mutant recipients exhibited a layered cellular organization reminiscent of the normal cerebellar cortex. Molecular, Purkinje, and granule cell layers were identifiable. Grafted Purkinje cells displayed characteristic cytological features, such as hypolemmal cisterns in association with mitochondria in the perikaryon, and lamellar structures in their axons. The cytological features of granule cell somata in the grafts appeared similar to those of mature granule cells. Electron microscopic examination of the molecular layer of the grafts revealed the presence of parallel fibers, which were not oriented in a parallel fashion; axon terminals of such fibers were often presynaptic to dendritic spines. The number of parallel fibers was markedly reduced in grafts implanted into both mutants compared to the normal cerebellar cortex; however, this phenomenon is commonly seen in cerebellum in tissue culture and in cerebellar transplants into normal hosts. It is concluded, therefore, that the environment of the mutant hosts does not affect the survival of Purkinje or granule cells and that transplantation of solid cerebellar grafts in the neurological mutants studied does not seem to pose any apparent limitations beyond those inherent to the process of cerebellar growth and differentiation outside its normal environment.

Original languageEnglish
Pages (from-to)145-154
Number of pages10
JournalAnatomy and Embryology
Volume176
Issue number2
DOIs
StatePublished - Jun 1987

Fingerprint

Atrophy
Transplantation
Transplants
Purkinje Cells
Cerebellar Cortex
Neurologic Mutant Mice
Dendritic Spines
Cell Transplantation
Presynaptic Terminals
Carisoprodol
Cerebellum
Axons
Mitochondria
Electrons
Growth

Keywords

  • 'Purkinje cell degeneration' (pcd)
  • Cerebellum
  • Electron microscopy
  • Light microscopy
  • Neural transplants
  • Neurological mutant mice
  • Weaver

ASJC Scopus subject areas

  • Embryology
  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Transplantation of cerebellar anlagen to hosts with genetic cerebellocortical atrophy. / Triarhou, Lazaros C.; Low, Walter C.; Ghetti, Bernardino.

In: Anatomy and Embryology, Vol. 176, No. 2, 06.1987, p. 145-154.

Research output: Contribution to journalArticle

@article{6bb80d7d4cbc4183bd113f5768e33dde,
title = "Transplantation of cerebellar anlagen to hosts with genetic cerebellocortical atrophy",
abstract = "Embryonic cerebellar grafts from genetically normal donors were implanted into the cerebellomedullary cistern of adult 'Purkinje cell degeneration' (pcd) and weaver mutant mice, which are respectively characterized by the selective loss of Purkinje and granule cells. Grafts placed into both mutant recipients exhibited a layered cellular organization reminiscent of the normal cerebellar cortex. Molecular, Purkinje, and granule cell layers were identifiable. Grafted Purkinje cells displayed characteristic cytological features, such as hypolemmal cisterns in association with mitochondria in the perikaryon, and lamellar structures in their axons. The cytological features of granule cell somata in the grafts appeared similar to those of mature granule cells. Electron microscopic examination of the molecular layer of the grafts revealed the presence of parallel fibers, which were not oriented in a parallel fashion; axon terminals of such fibers were often presynaptic to dendritic spines. The number of parallel fibers was markedly reduced in grafts implanted into both mutants compared to the normal cerebellar cortex; however, this phenomenon is commonly seen in cerebellum in tissue culture and in cerebellar transplants into normal hosts. It is concluded, therefore, that the environment of the mutant hosts does not affect the survival of Purkinje or granule cells and that transplantation of solid cerebellar grafts in the neurological mutants studied does not seem to pose any apparent limitations beyond those inherent to the process of cerebellar growth and differentiation outside its normal environment.",
keywords = "'Purkinje cell degeneration' (pcd), Cerebellum, Electron microscopy, Light microscopy, Neural transplants, Neurological mutant mice, Weaver",
author = "Triarhou, {Lazaros C.} and Low, {Walter C.} and Bernardino Ghetti",
year = "1987",
month = "6",
doi = "10.1007/BF00310047",
language = "English",
volume = "176",
pages = "145--154",
journal = "Anatomy and Embryology",
issn = "0177-5154",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Transplantation of cerebellar anlagen to hosts with genetic cerebellocortical atrophy

AU - Triarhou, Lazaros C.

AU - Low, Walter C.

AU - Ghetti, Bernardino

PY - 1987/6

Y1 - 1987/6

N2 - Embryonic cerebellar grafts from genetically normal donors were implanted into the cerebellomedullary cistern of adult 'Purkinje cell degeneration' (pcd) and weaver mutant mice, which are respectively characterized by the selective loss of Purkinje and granule cells. Grafts placed into both mutant recipients exhibited a layered cellular organization reminiscent of the normal cerebellar cortex. Molecular, Purkinje, and granule cell layers were identifiable. Grafted Purkinje cells displayed characteristic cytological features, such as hypolemmal cisterns in association with mitochondria in the perikaryon, and lamellar structures in their axons. The cytological features of granule cell somata in the grafts appeared similar to those of mature granule cells. Electron microscopic examination of the molecular layer of the grafts revealed the presence of parallel fibers, which were not oriented in a parallel fashion; axon terminals of such fibers were often presynaptic to dendritic spines. The number of parallel fibers was markedly reduced in grafts implanted into both mutants compared to the normal cerebellar cortex; however, this phenomenon is commonly seen in cerebellum in tissue culture and in cerebellar transplants into normal hosts. It is concluded, therefore, that the environment of the mutant hosts does not affect the survival of Purkinje or granule cells and that transplantation of solid cerebellar grafts in the neurological mutants studied does not seem to pose any apparent limitations beyond those inherent to the process of cerebellar growth and differentiation outside its normal environment.

AB - Embryonic cerebellar grafts from genetically normal donors were implanted into the cerebellomedullary cistern of adult 'Purkinje cell degeneration' (pcd) and weaver mutant mice, which are respectively characterized by the selective loss of Purkinje and granule cells. Grafts placed into both mutant recipients exhibited a layered cellular organization reminiscent of the normal cerebellar cortex. Molecular, Purkinje, and granule cell layers were identifiable. Grafted Purkinje cells displayed characteristic cytological features, such as hypolemmal cisterns in association with mitochondria in the perikaryon, and lamellar structures in their axons. The cytological features of granule cell somata in the grafts appeared similar to those of mature granule cells. Electron microscopic examination of the molecular layer of the grafts revealed the presence of parallel fibers, which were not oriented in a parallel fashion; axon terminals of such fibers were often presynaptic to dendritic spines. The number of parallel fibers was markedly reduced in grafts implanted into both mutants compared to the normal cerebellar cortex; however, this phenomenon is commonly seen in cerebellum in tissue culture and in cerebellar transplants into normal hosts. It is concluded, therefore, that the environment of the mutant hosts does not affect the survival of Purkinje or granule cells and that transplantation of solid cerebellar grafts in the neurological mutants studied does not seem to pose any apparent limitations beyond those inherent to the process of cerebellar growth and differentiation outside its normal environment.

KW - 'Purkinje cell degeneration' (pcd)

KW - Cerebellum

KW - Electron microscopy

KW - Light microscopy

KW - Neural transplants

KW - Neurological mutant mice

KW - Weaver

UR - http://www.scopus.com/inward/record.url?scp=0023222685&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023222685&partnerID=8YFLogxK

U2 - 10.1007/BF00310047

DO - 10.1007/BF00310047

M3 - Article

C2 - 3619071

AN - SCOPUS:0023222685

VL - 176

SP - 145

EP - 154

JO - Anatomy and Embryology

JF - Anatomy and Embryology

SN - 0177-5154

IS - 2

ER -