Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in λ phage

S. H. Phadnis, S. Kulakauskas, B. R. Krishnan, J. Hiemstra, D. E. Berg

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to λ-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the ability of insertion mutant phage to form haploid versus only heterozygous partial diploid bacterial recombinants.

Original languageEnglish (US)
Pages (from-to)896-899
Number of pages4
JournalJournal of bacteriology
Volume173
Issue number2
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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