Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in λ phage

S. H. Phadnis, S. Kulakauskas, B. R. Krishnan, Jill Reiter, D. E. Berg

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to λ-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the ability of insertion mutant phage to form haploid versus only heterozygous partial diploid bacterial recombinants.

Original languageEnglish (US)
Pages (from-to)896-899
Number of pages4
JournalJournal of Bacteriology
Volume173
Issue number2
StatePublished - 1991
Externally publishedYes

Fingerprint

Coliphages
Reverse Genetics
Haploidy
Essential Genes
Diploidy
Bacteriophages
Libraries
Clone Cells
Genome
Escherichia coli
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in λ phage. / Phadnis, S. H.; Kulakauskas, S.; Krishnan, B. R.; Reiter, Jill; Berg, D. E.

In: Journal of Bacteriology, Vol. 173, No. 2, 1991, p. 896-899.

Research output: Contribution to journalArticle

Phadnis, S. H. ; Kulakauskas, S. ; Krishnan, B. R. ; Reiter, Jill ; Berg, D. E. / Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in λ phage. In: Journal of Bacteriology. 1991 ; Vol. 173, No. 2. pp. 896-899.
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