Transretinal degeneration in ageing human retina: A multiphoton microscopy analysis

Y. Lei, N. Garrahan, B. Hermann, M. P. Fautsch, D. H. Johnson, M. R. Hernandez, M. Boulton, J. E. Morgan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aim: Retinal cell remodelling has been reported as a consistent feature of ageing. However, the degree to which this results in transretinal degeneration is unclear. To address this, the authors used multiphoton microscopy to quantify retinal degeneration in post-mortem human eyes of two age groups. Methods: Retinas from six young subjects (18-33 years old) and six older subjects (74-90 years old) were prepared as wholemount preparations. All retinas were stained with 4,6-diamidino-2-phenylindole and imaged by multiphoton confocal microscopy to quantify neuron densities in the retinal ganglion cell layer (RGCL), inner nuclear layer (INL) and outer nuclear layer (ONL). Neurons were counted using automated cell identification algorithms. All retinas were imaged hydrated to minimise tissue artefacts. Results: In both groups, 56% of the area within the central 4 mm eccentricity and 27% of the area with eccentricity between 4 mm and 7 mm were imaged. Compared with young subjects, the peak RGCL neuron loss in the aged subjects (25.5%) was at 1 mm eccentricity. INL and ONL neuron densities significantly decreased at 1-2 mm eccentricity (8.7%) and 0.5-4 mm eccentricity (15.6%) respectively (P

Original languageEnglish (US)
Pages (from-to)727-730
Number of pages4
JournalBritish Journal of Ophthalmology
Volume95
Issue number5
DOIs
StatePublished - May 2011
Externally publishedYes

Fingerprint

Retina
Microscopy
Neurons
Retinal Ganglion Cells
Retinal Degeneration
Confocal Microscopy
Artifacts
Age Groups

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Lei, Y., Garrahan, N., Hermann, B., Fautsch, M. P., Johnson, D. H., Hernandez, M. R., ... Morgan, J. E. (2011). Transretinal degeneration in ageing human retina: A multiphoton microscopy analysis. British Journal of Ophthalmology, 95(5), 727-730. https://doi.org/10.1136/bjo.2010.180869

Transretinal degeneration in ageing human retina : A multiphoton microscopy analysis. / Lei, Y.; Garrahan, N.; Hermann, B.; Fautsch, M. P.; Johnson, D. H.; Hernandez, M. R.; Boulton, M.; Morgan, J. E.

In: British Journal of Ophthalmology, Vol. 95, No. 5, 05.2011, p. 727-730.

Research output: Contribution to journalArticle

Lei, Y, Garrahan, N, Hermann, B, Fautsch, MP, Johnson, DH, Hernandez, MR, Boulton, M & Morgan, JE 2011, 'Transretinal degeneration in ageing human retina: A multiphoton microscopy analysis', British Journal of Ophthalmology, vol. 95, no. 5, pp. 727-730. https://doi.org/10.1136/bjo.2010.180869
Lei, Y. ; Garrahan, N. ; Hermann, B. ; Fautsch, M. P. ; Johnson, D. H. ; Hernandez, M. R. ; Boulton, M. ; Morgan, J. E. / Transretinal degeneration in ageing human retina : A multiphoton microscopy analysis. In: British Journal of Ophthalmology. 2011 ; Vol. 95, No. 5. pp. 727-730.
@article{91750a847146411ebf924828ce7be798,
title = "Transretinal degeneration in ageing human retina: A multiphoton microscopy analysis",
abstract = "Aim: Retinal cell remodelling has been reported as a consistent feature of ageing. However, the degree to which this results in transretinal degeneration is unclear. To address this, the authors used multiphoton microscopy to quantify retinal degeneration in post-mortem human eyes of two age groups. Methods: Retinas from six young subjects (18-33 years old) and six older subjects (74-90 years old) were prepared as wholemount preparations. All retinas were stained with 4,6-diamidino-2-phenylindole and imaged by multiphoton confocal microscopy to quantify neuron densities in the retinal ganglion cell layer (RGCL), inner nuclear layer (INL) and outer nuclear layer (ONL). Neurons were counted using automated cell identification algorithms. All retinas were imaged hydrated to minimise tissue artefacts. Results: In both groups, 56{\%} of the area within the central 4 mm eccentricity and 27{\%} of the area with eccentricity between 4 mm and 7 mm were imaged. Compared with young subjects, the peak RGCL neuron loss in the aged subjects (25.5{\%}) was at 1 mm eccentricity. INL and ONL neuron densities significantly decreased at 1-2 mm eccentricity (8.7{\%}) and 0.5-4 mm eccentricity (15.6{\%}) respectively (P",
author = "Y. Lei and N. Garrahan and B. Hermann and Fautsch, {M. P.} and Johnson, {D. H.} and Hernandez, {M. R.} and M. Boulton and Morgan, {J. E.}",
year = "2011",
month = "5",
doi = "10.1136/bjo.2010.180869",
language = "English (US)",
volume = "95",
pages = "727--730",
journal = "British Journal of Ophthalmology",
issn = "0007-1161",
publisher = "BMJ Publishing Group",
number = "5",

}

TY - JOUR

T1 - Transretinal degeneration in ageing human retina

T2 - A multiphoton microscopy analysis

AU - Lei, Y.

AU - Garrahan, N.

AU - Hermann, B.

AU - Fautsch, M. P.

AU - Johnson, D. H.

AU - Hernandez, M. R.

AU - Boulton, M.

AU - Morgan, J. E.

PY - 2011/5

Y1 - 2011/5

N2 - Aim: Retinal cell remodelling has been reported as a consistent feature of ageing. However, the degree to which this results in transretinal degeneration is unclear. To address this, the authors used multiphoton microscopy to quantify retinal degeneration in post-mortem human eyes of two age groups. Methods: Retinas from six young subjects (18-33 years old) and six older subjects (74-90 years old) were prepared as wholemount preparations. All retinas were stained with 4,6-diamidino-2-phenylindole and imaged by multiphoton confocal microscopy to quantify neuron densities in the retinal ganglion cell layer (RGCL), inner nuclear layer (INL) and outer nuclear layer (ONL). Neurons were counted using automated cell identification algorithms. All retinas were imaged hydrated to minimise tissue artefacts. Results: In both groups, 56% of the area within the central 4 mm eccentricity and 27% of the area with eccentricity between 4 mm and 7 mm were imaged. Compared with young subjects, the peak RGCL neuron loss in the aged subjects (25.5%) was at 1 mm eccentricity. INL and ONL neuron densities significantly decreased at 1-2 mm eccentricity (8.7%) and 0.5-4 mm eccentricity (15.6%) respectively (P

AB - Aim: Retinal cell remodelling has been reported as a consistent feature of ageing. However, the degree to which this results in transretinal degeneration is unclear. To address this, the authors used multiphoton microscopy to quantify retinal degeneration in post-mortem human eyes of two age groups. Methods: Retinas from six young subjects (18-33 years old) and six older subjects (74-90 years old) were prepared as wholemount preparations. All retinas were stained with 4,6-diamidino-2-phenylindole and imaged by multiphoton confocal microscopy to quantify neuron densities in the retinal ganglion cell layer (RGCL), inner nuclear layer (INL) and outer nuclear layer (ONL). Neurons were counted using automated cell identification algorithms. All retinas were imaged hydrated to minimise tissue artefacts. Results: In both groups, 56% of the area within the central 4 mm eccentricity and 27% of the area with eccentricity between 4 mm and 7 mm were imaged. Compared with young subjects, the peak RGCL neuron loss in the aged subjects (25.5%) was at 1 mm eccentricity. INL and ONL neuron densities significantly decreased at 1-2 mm eccentricity (8.7%) and 0.5-4 mm eccentricity (15.6%) respectively (P

UR - http://www.scopus.com/inward/record.url?scp=79955030808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955030808&partnerID=8YFLogxK

U2 - 10.1136/bjo.2010.180869

DO - 10.1136/bjo.2010.180869

M3 - Article

C2 - 21183516

AN - SCOPUS:79955030808

VL - 95

SP - 727

EP - 730

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

SN - 0007-1161

IS - 5

ER -