Trans,trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties

Aline Rogéria Freire De Castilho, Pedro Luiz Rosalen, Isaac Jordão De Souza Araújo, Igor Lebedenco Kitagawa, Cecilia Atem Gonçalves De Araújo Costa, Malvin N. Janal, Marcelo Corrêa Alves, Simone Duarte, Paulo Noronha Lisboa Filho, Rafael Nobrega Stipp, Regina Maria Puppin-Rontani

Research output: Contribution to journalArticle

Abstract

A series of experiments were conducted to characterize a novel restorative material. We explored the effect on biological, physical and chemical properties of glass ionomer cement (GIC) adding-the naturally occurring tt-farnesol (900 mM). Two groups were accomplished for all assays: GIC+tt-farnesol and GIC (control). Biological assays: 1) agar diffusion against some cariogenic bacteria; 2) S. mutans biofilm formation and confocal laser scanning microscopy-CLSM. 3) gtfB, gtfC, gtfD, gbpB, vicR, and covR expression; 4) MTT and microscopic morphology. Physical properties assays: 1) roughness; 2) hardness; 3) compressive strength and 4) diametral tensile strength. Chemical assay: Raman spectroscopy. The adding of tt-farnesol to GIC led to larger zones of inhibition (p<0.05), biofilms with a short-term reduction in bacterial viability but similar biomass (p>0.05). Polysaccharides levels increased over time, similarly over groups (p>0.05). Viable and non-viable S. mutans were seen on the specimens’ surface by CLSM but their virulence was not modulated by tt-farnesol. The tt-farnesol increased the HaCaT cell viability without impact on compressive and diametral tensile strength and roughness although the hardness was positively affected (p<0.05). Raman confirmed the presence of tt-farnesol. The incorporation of tt-farnesol into GIC inhibited the growth of cariogenic bacteria but had a little effect on the composition, structure and physiology of the biofilm matrices. Also, the tt-farnesol increased the hardness and the biocompatibility of the GIC, not influencing negatively other physical properties of the restorative material.

Original languageEnglish (US)
Article numbere0220718
JournalPLoS ONE
Volume14
Issue number8
DOIs
StatePublished - Jan 1 2019

Fingerprint

Farnesol
farnesol
Glass Ionomer Cements
cement
glass
anti-infective agents
Assays
Hardness
Biofilms
biofilm
hardness
physical properties
Physical properties
Tensile Strength
roughness
tensile strength
Bacteria
assays
Tensile strength
Surface roughness

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

De Castilho, A. R. F., Rosalen, P. L., De Souza Araújo, I. J., Kitagawa, I. L., De Araújo Costa, C. A. G., Janal, M. N., ... Puppin-Rontani, R. M. (2019). Trans,trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties. PLoS ONE, 14(8), [e0220718]. https://doi.org/10.1371/journal.pone.0220718

Trans,trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties. / De Castilho, Aline Rogéria Freire; Rosalen, Pedro Luiz; De Souza Araújo, Isaac Jordão; Kitagawa, Igor Lebedenco; De Araújo Costa, Cecilia Atem Gonçalves; Janal, Malvin N.; Alves, Marcelo Corrêa; Duarte, Simone; Filho, Paulo Noronha Lisboa; Stipp, Rafael Nobrega; Puppin-Rontani, Regina Maria.

In: PLoS ONE, Vol. 14, No. 8, e0220718, 01.01.2019.

Research output: Contribution to journalArticle

De Castilho, ARF, Rosalen, PL, De Souza Araújo, IJ, Kitagawa, IL, De Araújo Costa, CAG, Janal, MN, Alves, MC, Duarte, S, Filho, PNL, Stipp, RN & Puppin-Rontani, RM 2019, 'Trans,trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties', PLoS ONE, vol. 14, no. 8, e0220718. https://doi.org/10.1371/journal.pone.0220718
De Castilho ARF, Rosalen PL, De Souza Araújo IJ, Kitagawa IL, De Araújo Costa CAG, Janal MN et al. Trans,trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties. PLoS ONE. 2019 Jan 1;14(8). e0220718. https://doi.org/10.1371/journal.pone.0220718
De Castilho, Aline Rogéria Freire ; Rosalen, Pedro Luiz ; De Souza Araújo, Isaac Jordão ; Kitagawa, Igor Lebedenco ; De Araújo Costa, Cecilia Atem Gonçalves ; Janal, Malvin N. ; Alves, Marcelo Corrêa ; Duarte, Simone ; Filho, Paulo Noronha Lisboa ; Stipp, Rafael Nobrega ; Puppin-Rontani, Regina Maria. / Trans,trans-farnesol, an antimicrobial natural compound, improves glass ionomer cement properties. In: PLoS ONE. 2019 ; Vol. 14, No. 8.
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