Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery

Effects on Dual Species Biofilm and Cell Function

Divya Pankajakshan, Maria T P Albuquerque, Joshua D. Evans, Malgorzata Kamocka, Richard Gregory, Marco C. Bottino

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Introduction Root canal disinfection and the establishment of an intracanal microenvironment conducive to the proliferation/differentiation of stem cells play a significant role in regenerative endodontics. This study was designed to (1) investigate the antimicrobial efficacy of triple antibiotic–containing nanofibers against a dual-species biofilm and (2) evaluate the ability of dental pulp stem cells (DPSCs) to adhere to and proliferate on dentin upon nanofiber exposure. Methods Seven-day-old dual-species biofilm established on dentin specimens was exposed for 3 days to the following: saline (control), antibiotic-free nanofibers (control), and triple antibiotic–containing nanofibers or a saturated triple antibiotic paste (TAP) solution (50 mg/mL in phosphate buffer solution). Bacterial viability was assessed using the LIVE/DEAD assay (Molecular Probes, Inc, Eugene, OR) and confocal laser scanning microscopy. For cytocompatibility studies, dentin specimens after nanofiber or TAP (1 g/mL in phosphate buffer solution) exposure were evaluated for cell adhesion and spreading by actin-phalloidin staining. DPSC proliferation was assessed on days 1, 3, and 7. Statistics were performed, and significance was set at the 5% level. Results Confocal laser scanning microscopy showed significant bacterial death upon antibiotic-containing nanofiber exposure, differing significantly (P < .05) from antibiotic-free fibers and the control (saline). DPSCs showed enhanced adhesion/spreading on dentin specimens treated with antibiotic-containing nanofibers when compared with its TAP counterparts. The DPSC proliferation rate was similar on days 1 and 3 in antibiotic-free nanofibers, triple antibiotic–containing nanofibers, and TAP-treated dentin. Proliferation was higher (9-fold) on dentin treated with antibiotic-containing nanofibers on day 7 compared with TAP. Conclusions Triple antibiotic–containing polymer nanofibers led to significant bacterial death, whereas they did not affect DPSC attachment and proliferation on dentin.

Original languageEnglish (US)
Pages (from-to)1490-1495
Number of pages6
JournalJournal of Endodontics
Volume42
Issue number10
DOIs
StatePublished - Oct 1 2016

Fingerprint

Nanofibers
Biofilms
Polymers
Anti-Bacterial Agents
Dentin
Dental Pulp
Pharmaceutical Preparations
Ointments
Stem Cells
Cell Proliferation
Confocal Microscopy
Buffers
Phosphates
Microbial Viability
Phalloidine
Molecular Probes
Dental Pulp Cavity
Endodontics
Disinfection
Cell Adhesion

Keywords

  • Antibiotic
  • disinfection
  • electrospinning
  • nanofibers
  • regeneration
  • stem cells

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery : Effects on Dual Species Biofilm and Cell Function. / Pankajakshan, Divya; Albuquerque, Maria T P; Evans, Joshua D.; Kamocka, Malgorzata; Gregory, Richard; Bottino, Marco C.

In: Journal of Endodontics, Vol. 42, No. 10, 01.10.2016, p. 1490-1495.

Research output: Contribution to journalArticle

Pankajakshan, Divya ; Albuquerque, Maria T P ; Evans, Joshua D. ; Kamocka, Malgorzata ; Gregory, Richard ; Bottino, Marco C. / Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery : Effects on Dual Species Biofilm and Cell Function. In: Journal of Endodontics. 2016 ; Vol. 42, No. 10. pp. 1490-1495.
@article{e7f92daed2334f84b6805041b5d59d1a,
title = "Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function",
abstract = "Introduction Root canal disinfection and the establishment of an intracanal microenvironment conducive to the proliferation/differentiation of stem cells play a significant role in regenerative endodontics. This study was designed to (1) investigate the antimicrobial efficacy of triple antibiotic–containing nanofibers against a dual-species biofilm and (2) evaluate the ability of dental pulp stem cells (DPSCs) to adhere to and proliferate on dentin upon nanofiber exposure. Methods Seven-day-old dual-species biofilm established on dentin specimens was exposed for 3 days to the following: saline (control), antibiotic-free nanofibers (control), and triple antibiotic–containing nanofibers or a saturated triple antibiotic paste (TAP) solution (50 mg/mL in phosphate buffer solution). Bacterial viability was assessed using the LIVE/DEAD assay (Molecular Probes, Inc, Eugene, OR) and confocal laser scanning microscopy. For cytocompatibility studies, dentin specimens after nanofiber or TAP (1 g/mL in phosphate buffer solution) exposure were evaluated for cell adhesion and spreading by actin-phalloidin staining. DPSC proliferation was assessed on days 1, 3, and 7. Statistics were performed, and significance was set at the 5{\%} level. Results Confocal laser scanning microscopy showed significant bacterial death upon antibiotic-containing nanofiber exposure, differing significantly (P < .05) from antibiotic-free fibers and the control (saline). DPSCs showed enhanced adhesion/spreading on dentin specimens treated with antibiotic-containing nanofibers when compared with its TAP counterparts. The DPSC proliferation rate was similar on days 1 and 3 in antibiotic-free nanofibers, triple antibiotic–containing nanofibers, and TAP-treated dentin. Proliferation was higher (9-fold) on dentin treated with antibiotic-containing nanofibers on day 7 compared with TAP. Conclusions Triple antibiotic–containing polymer nanofibers led to significant bacterial death, whereas they did not affect DPSC attachment and proliferation on dentin.",
keywords = "Antibiotic, disinfection, electrospinning, nanofibers, regeneration, stem cells",
author = "Divya Pankajakshan and Albuquerque, {Maria T P} and Evans, {Joshua D.} and Malgorzata Kamocka and Richard Gregory and Bottino, {Marco C.}",
year = "2016",
month = "10",
day = "1",
doi = "10.1016/j.joen.2016.07.019",
language = "English (US)",
volume = "42",
pages = "1490--1495",
journal = "Journal of Endodontics",
issn = "0099-2399",
publisher = "Elsevier Inc.",
number = "10",

}

TY - JOUR

T1 - Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery

T2 - Effects on Dual Species Biofilm and Cell Function

AU - Pankajakshan, Divya

AU - Albuquerque, Maria T P

AU - Evans, Joshua D.

AU - Kamocka, Malgorzata

AU - Gregory, Richard

AU - Bottino, Marco C.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Introduction Root canal disinfection and the establishment of an intracanal microenvironment conducive to the proliferation/differentiation of stem cells play a significant role in regenerative endodontics. This study was designed to (1) investigate the antimicrobial efficacy of triple antibiotic–containing nanofibers against a dual-species biofilm and (2) evaluate the ability of dental pulp stem cells (DPSCs) to adhere to and proliferate on dentin upon nanofiber exposure. Methods Seven-day-old dual-species biofilm established on dentin specimens was exposed for 3 days to the following: saline (control), antibiotic-free nanofibers (control), and triple antibiotic–containing nanofibers or a saturated triple antibiotic paste (TAP) solution (50 mg/mL in phosphate buffer solution). Bacterial viability was assessed using the LIVE/DEAD assay (Molecular Probes, Inc, Eugene, OR) and confocal laser scanning microscopy. For cytocompatibility studies, dentin specimens after nanofiber or TAP (1 g/mL in phosphate buffer solution) exposure were evaluated for cell adhesion and spreading by actin-phalloidin staining. DPSC proliferation was assessed on days 1, 3, and 7. Statistics were performed, and significance was set at the 5% level. Results Confocal laser scanning microscopy showed significant bacterial death upon antibiotic-containing nanofiber exposure, differing significantly (P < .05) from antibiotic-free fibers and the control (saline). DPSCs showed enhanced adhesion/spreading on dentin specimens treated with antibiotic-containing nanofibers when compared with its TAP counterparts. The DPSC proliferation rate was similar on days 1 and 3 in antibiotic-free nanofibers, triple antibiotic–containing nanofibers, and TAP-treated dentin. Proliferation was higher (9-fold) on dentin treated with antibiotic-containing nanofibers on day 7 compared with TAP. Conclusions Triple antibiotic–containing polymer nanofibers led to significant bacterial death, whereas they did not affect DPSC attachment and proliferation on dentin.

AB - Introduction Root canal disinfection and the establishment of an intracanal microenvironment conducive to the proliferation/differentiation of stem cells play a significant role in regenerative endodontics. This study was designed to (1) investigate the antimicrobial efficacy of triple antibiotic–containing nanofibers against a dual-species biofilm and (2) evaluate the ability of dental pulp stem cells (DPSCs) to adhere to and proliferate on dentin upon nanofiber exposure. Methods Seven-day-old dual-species biofilm established on dentin specimens was exposed for 3 days to the following: saline (control), antibiotic-free nanofibers (control), and triple antibiotic–containing nanofibers or a saturated triple antibiotic paste (TAP) solution (50 mg/mL in phosphate buffer solution). Bacterial viability was assessed using the LIVE/DEAD assay (Molecular Probes, Inc, Eugene, OR) and confocal laser scanning microscopy. For cytocompatibility studies, dentin specimens after nanofiber or TAP (1 g/mL in phosphate buffer solution) exposure were evaluated for cell adhesion and spreading by actin-phalloidin staining. DPSC proliferation was assessed on days 1, 3, and 7. Statistics were performed, and significance was set at the 5% level. Results Confocal laser scanning microscopy showed significant bacterial death upon antibiotic-containing nanofiber exposure, differing significantly (P < .05) from antibiotic-free fibers and the control (saline). DPSCs showed enhanced adhesion/spreading on dentin specimens treated with antibiotic-containing nanofibers when compared with its TAP counterparts. The DPSC proliferation rate was similar on days 1 and 3 in antibiotic-free nanofibers, triple antibiotic–containing nanofibers, and TAP-treated dentin. Proliferation was higher (9-fold) on dentin treated with antibiotic-containing nanofibers on day 7 compared with TAP. Conclusions Triple antibiotic–containing polymer nanofibers led to significant bacterial death, whereas they did not affect DPSC attachment and proliferation on dentin.

KW - Antibiotic

KW - disinfection

KW - electrospinning

KW - nanofibers

KW - regeneration

KW - stem cells

UR - http://www.scopus.com/inward/record.url?scp=84990189858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990189858&partnerID=8YFLogxK

U2 - 10.1016/j.joen.2016.07.019

DO - 10.1016/j.joen.2016.07.019

M3 - Article

VL - 42

SP - 1490

EP - 1495

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 10

ER -