Trisomy 7-harbouring non-random duplication of the mutant MET allele in hereditary papillary renal carcinomas

Zhengping Zhuang, Won Sang Park, Svetlana Pack, Laura Schmidt, Alexander Vortmeyer, Evgenia Pak, Thu Pham, Robert J. Weil, Sonja Candidas, Irina A. Lubensky, W. Marston Linehan, Berton Zbar, Gregor Weirich

Research output: Contribution to journalArticle

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Abstract

The gene defect for hereditary papillary renal carcinoma1 (HPRC) has recently been mapped to chromosome 7q, and germline mutations of MET (also known as c-met) at 7q31 have been detected in patients with HPRC (ref. 2). Tumours from these patients commonly show trisomy of chromosome 7 when analysed by cytogenetic studies and comparative genomic hybridization3 (CGH). However, the relationship between trisomy 7 and MET germline mutations is not clear. We studied 16 renal tumours from two patients with documented germline mutations in exon 16 of MET. Flourescent in situ hybridization (FISH) analysis showed trisomy 7 in all tumours. To determine whether the chromosome bearing the mutant or wild-type MET gene was duplicated, we performed duplex PCR and phosphoimage densitometry using polymorphic microsatellite markers D7S1801 and D7S1822, which were linked to the disease gene locus, and DIS1646 as an internal control. We determined the parental origin of chromosome alleles by genotyping parental DNA. In all 16 tumours there was an increased signal intensity (2:1 ratio) of the microsatellite allele from the chromosome bearing the mutant MET compared with the allele from the chromosome bearing the wild-type MET. Our study demonstrates a non- random duplication of the chromosome bearing the mutated MET in HPRC and implicates this event in tumorigenesis.

Original languageEnglish (US)
Pages (from-to)66-69
Number of pages4
JournalNature genetics
Volume20
Issue number1
DOIs
StatePublished - Sep 15 1998
Externally publishedYes

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Papillary Carcinoma
Trisomy
Chromosomes
Alleles
Germ-Line Mutation
Kidney
Microsatellite Repeats
Neoplasms
Chromosome Duplication
Genes
Chromosomes, Human, Pair 7
Densitometry
Cytogenetics
In Situ Hybridization
Exons
Carcinogenesis
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Trisomy 7-harbouring non-random duplication of the mutant MET allele in hereditary papillary renal carcinomas. / Zhuang, Zhengping; Park, Won Sang; Pack, Svetlana; Schmidt, Laura; Vortmeyer, Alexander; Pak, Evgenia; Pham, Thu; Weil, Robert J.; Candidas, Sonja; Lubensky, Irina A.; Marston Linehan, W.; Zbar, Berton; Weirich, Gregor.

In: Nature genetics, Vol. 20, No. 1, 15.09.1998, p. 66-69.

Research output: Contribution to journalArticle

Zhuang, Z, Park, WS, Pack, S, Schmidt, L, Vortmeyer, A, Pak, E, Pham, T, Weil, RJ, Candidas, S, Lubensky, IA, Marston Linehan, W, Zbar, B & Weirich, G 1998, 'Trisomy 7-harbouring non-random duplication of the mutant MET allele in hereditary papillary renal carcinomas', Nature genetics, vol. 20, no. 1, pp. 66-69. https://doi.org/10.1038/1727
Zhuang, Zhengping ; Park, Won Sang ; Pack, Svetlana ; Schmidt, Laura ; Vortmeyer, Alexander ; Pak, Evgenia ; Pham, Thu ; Weil, Robert J. ; Candidas, Sonja ; Lubensky, Irina A. ; Marston Linehan, W. ; Zbar, Berton ; Weirich, Gregor. / Trisomy 7-harbouring non-random duplication of the mutant MET allele in hereditary papillary renal carcinomas. In: Nature genetics. 1998 ; Vol. 20, No. 1. pp. 66-69.
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