TSH controls Ref-1 nuclear translocation in thyroid cells

G. Tell, L. Pellizzari, C. Pucillo, F. Puglisi, D. Cesselli, M. R. Kelley, C. Di Loreto, G. Damante

Research output: Contribution to journalArticle

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Abstract

Ref-1 (called also APE) is a bifunctional protein playing a role in a large variety of cell functions. It is a major member of the DNA base excision repair system. Moreover, through reduction of cysteine residues, Ref-1 controls the activity of several transcription factors. It has been previously demonstrated that TSH up-regulates Ref-1 gene expression in thyroid cells. By using the rat FRTL-5 cell line, we demonstrate that TSH controls Ref-1 intracellular localization. Western blot experiments indicate that addition of TSH to the culture medium increases the Ref-1 cytoplasm-to- nucleus translocation. This phenomenon occurs at early times of TSH stimulation and is not dependent on protein neosynthesis. The Ref-1 cellular compartmentalization was also investigated in human thyroid tumors. A Ref-1 nuclear/cytoplasmic ratio difference between normal and cancerous thyroid tissues was observed. These results suggest that Ref-1 localization may have a critical role in the control of thyroid cell functions.

Original languageEnglish (US)
Pages (from-to)383-390
Number of pages8
JournalJournal of Molecular Endocrinology
Volume24
Issue number3
DOIs
StatePublished - Jun 2000

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Thyroid Gland
DNA Repair
Cysteine
Culture Media
Cytoplasm
Proteins
Transcription Factors
Up-Regulation
Western Blotting
Gene Expression
Cell Line
DNA
Neoplasms

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Tell, G., Pellizzari, L., Pucillo, C., Puglisi, F., Cesselli, D., Kelley, M. R., ... Damante, G. (2000). TSH controls Ref-1 nuclear translocation in thyroid cells. Journal of Molecular Endocrinology, 24(3), 383-390. https://doi.org/10.1677/jme.0.0240383

TSH controls Ref-1 nuclear translocation in thyroid cells. / Tell, G.; Pellizzari, L.; Pucillo, C.; Puglisi, F.; Cesselli, D.; Kelley, M. R.; Di Loreto, C.; Damante, G.

In: Journal of Molecular Endocrinology, Vol. 24, No. 3, 06.2000, p. 383-390.

Research output: Contribution to journalArticle

Tell, G, Pellizzari, L, Pucillo, C, Puglisi, F, Cesselli, D, Kelley, MR, Di Loreto, C & Damante, G 2000, 'TSH controls Ref-1 nuclear translocation in thyroid cells', Journal of Molecular Endocrinology, vol. 24, no. 3, pp. 383-390. https://doi.org/10.1677/jme.0.0240383
Tell, G. ; Pellizzari, L. ; Pucillo, C. ; Puglisi, F. ; Cesselli, D. ; Kelley, M. R. ; Di Loreto, C. ; Damante, G. / TSH controls Ref-1 nuclear translocation in thyroid cells. In: Journal of Molecular Endocrinology. 2000 ; Vol. 24, No. 3. pp. 383-390.
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