TSH controls Ref-1 nuclear translocation in thyroid cells

G. Tell, L. Pellizzari, C. Pucillo, F. Puglisi, D. Cesselli, M. R. Kelley, C. Di Loreto, G. Damante

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

Ref-1 (called also APE) is a bifunctional protein playing a role in a large variety of cell functions. It is a major member of the DNA base excision repair system. Moreover, through reduction of cysteine residues, Ref-1 controls the activity of several transcription factors. It has been previously demonstrated that TSH up-regulates Ref-1 gene expression in thyroid cells. By using the rat FRTL-5 cell line, we demonstrate that TSH controls Ref-1 intracellular localization. Western blot experiments indicate that addition of TSH to the culture medium increases the Ref-1 cytoplasm-to- nucleus translocation. This phenomenon occurs at early times of TSH stimulation and is not dependent on protein neosynthesis. The Ref-1 cellular compartmentalization was also investigated in human thyroid tumors. A Ref-1 nuclear/cytoplasmic ratio difference between normal and cancerous thyroid tissues was observed. These results suggest that Ref-1 localization may have a critical role in the control of thyroid cell functions.

Original languageEnglish (US)
Pages (from-to)383-390
Number of pages8
JournalJournal of Molecular Endocrinology
Volume24
Issue number3
DOIs
StatePublished - Jun 2000

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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    Tell, G., Pellizzari, L., Pucillo, C., Puglisi, F., Cesselli, D., Kelley, M. R., Di Loreto, C., & Damante, G. (2000). TSH controls Ref-1 nuclear translocation in thyroid cells. Journal of Molecular Endocrinology, 24(3), 383-390. https://doi.org/10.1677/jme.0.0240383