TT virus: Preferential distribution in CD19+ peripheral blood mononuclear cells and lack of viral integration

Andy Yu, Bruce Shiramizu, Vivek R. Nerurkar, Ningjie Hu, Cecilia M. Shikuma, Marian E. Melish, Karin Cascio, Allison Imrie, Yuanan Lu, Richard Yanagihara

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double-stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer sets. TTV sequences were detected in nearly 20%(25/126) of PBMC DNA samples. Analysis of PBMC subsets revealed that TTV was preferentially distributed in CD19+ cells. TTV DNA was also detected in CD3+/CD19+ double-depleted cells but was undetectable in CD3+ cells. After degradation of single-stranded DNA using mung bean nuclease (MBN), TTV could not be detected in previously TTV-positive PBMC DNA samples nor in DNA from CD19+ cells, CD3+-depleted, and CD3+/CD19+ double-depleted cells, indicating the lack of TTV DNA integration and the absence of viral double-stranded replicative intermediates in PBMC or PBMC subset cells. Our data indicate that PBMC and PBMC subsets are not the major sites of TTV replication.

Original languageEnglish (US)
Pages (from-to)276-284
Number of pages9
JournalJournal of Medical Virology
Volume66
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Torque teno virus
Virus Integration
Blood Cells
DNA
Single-Stranded DNA
Virus Replication

Keywords

  • CD19 cell
  • CD3 cell
  • Circovirus
  • Peripheral blood mononuclear cell
  • Polymerase chain reaction
  • Replication
  • TT virus

ASJC Scopus subject areas

  • Virology

Cite this

Yu, A., Shiramizu, B., Nerurkar, V. R., Hu, N., Shikuma, C. M., Melish, M. E., ... Yanagihara, R. (2002). TT virus: Preferential distribution in CD19+ peripheral blood mononuclear cells and lack of viral integration. Journal of Medical Virology, 66(2), 276-284. https://doi.org/10.1002/jmv.2142

TT virus : Preferential distribution in CD19+ peripheral blood mononuclear cells and lack of viral integration. / Yu, Andy; Shiramizu, Bruce; Nerurkar, Vivek R.; Hu, Ningjie; Shikuma, Cecilia M.; Melish, Marian E.; Cascio, Karin; Imrie, Allison; Lu, Yuanan; Yanagihara, Richard.

In: Journal of Medical Virology, Vol. 66, No. 2, 2002, p. 276-284.

Research output: Contribution to journalArticle

Yu, A, Shiramizu, B, Nerurkar, VR, Hu, N, Shikuma, CM, Melish, ME, Cascio, K, Imrie, A, Lu, Y & Yanagihara, R 2002, 'TT virus: Preferential distribution in CD19+ peripheral blood mononuclear cells and lack of viral integration', Journal of Medical Virology, vol. 66, no. 2, pp. 276-284. https://doi.org/10.1002/jmv.2142
Yu, Andy ; Shiramizu, Bruce ; Nerurkar, Vivek R. ; Hu, Ningjie ; Shikuma, Cecilia M. ; Melish, Marian E. ; Cascio, Karin ; Imrie, Allison ; Lu, Yuanan ; Yanagihara, Richard. / TT virus : Preferential distribution in CD19+ peripheral blood mononuclear cells and lack of viral integration. In: Journal of Medical Virology. 2002 ; Vol. 66, No. 2. pp. 276-284.
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